Enzymatic treatments have grown in popularity in the textile sector due to their energy-saving, non-toxic, stereo-specific, and environmentally friendliness. Almost every production step of the wet processing of textiles can benefit from the use of enzymes starting from the fiber processing to modify or functionalize the textile surface. Given the high demand of the enzyme and the need to reuse of enzyme while increasing their stability of enzyme in various processing condition, the framework of immobilized enzyme has been introduced. Immobilized enzyme has found promising application to the various textile processing, that includes desizing, scouring, bleaching, biopolishing, washing, surface modification as well as textile effluent treatment. The chapter presents the progresses and developments in use of immobilized enzyme in textile processing with a key focus on the fundamentals of enzyme immobilization, industrial applications of enzyme in textile processing, application of immobilized enzymes in textile processing as well as challenges and future perspectives immobilized enzyme.