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Use of a coaxial nozzle-assisted 3D printing and ionic crosslinking to control the texture of a mycoprotein-based food product
Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal; International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga, Braga, 4715-330, Portugal; LABBELS - Associate Laboratory, Braga/Guimarães, Portugal.ORCID iD: 0000-0002-5364-602X
International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga, Braga, 4715-330, Portugal.
International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga, Braga, 4715-330, Portugal.
International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga, Braga, 4715-330, Portugal.
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2025 (English)In: Future Foods, ISSN 2666-8335, Vol. 11, article id 100530Article in journal (Refereed) Published
Sustainable development
According to the author(s), the content of this publication falls within the area of sustainable development.
Abstract [en]

This work innovatively merges engineered food structures with additive manufacturing technology to tailor food for different dysphagia levels using filamentous Fungus Aspergillus oryzae biomass through a co-axial 3D food printing process. In order to 3D print samples with different textures, calcium chloride (CaCl2) and calcium gluconolactate (Gluco) were tested at different concentrations alongside three alginate concentrations. The structural and morphological analysis of the printed samples was conducted before cooking, followed by texture analysis and the measurement of cutting strength on cooked samples. Dysphagia food level classification was measured following the International Dysphagia Diet Standardisation Initiative (IDDSI) guidelines. Results revealed that Gluco minimally altered texture but affected appearance, resulting in soft samples (hardness ≈ 1.3 N) with fibrous structures, while CaCl2 increased hardness (from ≈ 1 N to 4.68 N) through alginate-ion Ca2+ interaction. All samples passed the IDDSI test, confirming suitability for dysphagic individuals. This successfully engineered personalised food with tailored texture meeting nutritional requirements. 

Place, publisher, year, edition, pages
2025. Vol. 11, article id 100530
Keywords [en]
3D food printing, alternative proteins, dysphagia food, mycoprotein, texture personalisation
National Category
Food Science
Research subject
Resource Recovery
Identifiers
URN: urn:nbn:se:hb:diva-33033DOI: 10.1016/j.fufo.2024.100530ISI: 001403226600001Scopus ID: 2-s2.0-85213288676OAI: oai:DiVA.org:hb-33033DiVA, id: diva2:1925152
Note

The author Arlete M. Marques (SFRH/BD/132911/2017) is the recipient of a fellowship from Funda\u00E7\u00E3o para a Ci\u00EAncia e Tecnologia (FCT, Portugal). Ana Isabel Bourbon acknowledges funding by FCT through the individual scientific employment program contract (2020.03447.CEECIND). This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit, and by LABBELS \u2013 Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020. We also would like to thank the Advanced Electron Microscopy, Imaging, and Spectroscopy (AEMIS) and Nanophotonics and Bioimaging (NBI) from INL for their support.

Available from: 2025-01-08 Created: 2025-01-08 Last updated: 2026-01-30Bibliographically approved

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Rousta, NedaTaherzadeh, Mohammad J

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