The production methods could affect the properties of the components produced. This study investigatesthe impact of a production method, 3D printer, on water absorption, mechanical and thermal propertiesof the biocomposites produced from poly(lactic) acid and wood particles. The results were analyzedcomparatively with injection molding. A minor change (0.1 mm) in the layer thickness in 3D printing affectedthe biocomposites’ properties significantly. The water absorption in 3D printed biocomposites causedswelling leading to permanent dimensional changes and initiated many failure nodes across layers. Thewater absorption of the injection molded biocomposites was primarily due to the material properties whilethe water absorption of the 3D printed biocomposites was due to the combination of intrinsic porosity ofthe 3D printing and the material properties. Water absorption in injected molded biocomposites was dueto the gradual absorption of water from surface to the core. Increasing the biocomposites’ surface contactwith water in 3D printing increased the water absorption. Additionally, increasing the layer thickness in 3Dprinting increased the water absorption further. 3D printed biocomposites mechanically performed betteron decreasing the layer thickness. Lower porosity on decreasing the layer thickness was the predominantfactor. A structure’s overall strength increased when there were more contact sites and greater interlayerbonding due to smaller layer thickness. Injection molded biocomposites had higher density and bettermechanical properties than 3D printed biocomposites due to solid specimens resulting in reduced failuresites. The results from this comparative study highlights the limitations of 3D printing.