Food loss and waste (FLW) occurring early in the food supply chain (FSC) leads to increased resource wastage, including land, water, fertilisers, pesticides, fuel, packaging, energy, and labour. Targeting FLW prevention benefits various aspects such as food security, productivity, economic growth, climate change mitigation, resource conservation, and food waste management. Understanding the causes of FLW and their environmental impact is crucial for the design of effective solutions and their prioritisation. The purpose of this study was to investigate the risk factors and underlying causes of FLW in leafy vegetables (LV), mainly lettuce, throughout the Brazilian FSC, spanning from harvest to retail. Additionally, the study evaluated the environmental impact of waste management strategies applicable in the context of the case study. To achieve this, the research methodology encompassed a case study conducted among small-scale producers and retailers in the city of Tupã, Brazil. A comprehensive approach was adopted by integrating a systematic literature review of global FLW causes and those specific to the Latin American context. This approach was complemented by exploratory research, involving interviews with various stakeholders along the FSC, coupled with rigorous root-cause analysis. Moreover, the study employed a Life Cycle Assessment (LCA) methodology to offer an immersive perspective, determining the environmental implications associated with different approaches to treating lettuce waste in the case study. Findings revealed that the root causes of FLW in the case study encompassed normalised unfair trading practices, notably take-back agreement (TBA) conditions, and the absence of supportive policies and incentives for FLW reduction. These root causes manifested in the absence of formal agreements between retailers and local producers, leading to the return of unsold or substandard items without compensation. Furthermore, supermarkets exert power over product quality but evade responsibility for proper storage or encouraging the utilisation of unsold products. Another category of significant causes, designated by the author as "major causes," encompassed causes such as unpreparedness for adverse weather conditions, lack of skilled labour, and stringent visual quality standards. These factors were pivotal risk contributors that potentially motivate various other causes of FLW. To tackle root and major causes of FLW of LV, this study proposed specific measures encompassing fair trade agreements, policy enhancements, protective measures for producers, skill development, and flexible standards. Moreover, by implementing an attributional LCA methodology, the study underscored the importance of source reduction in preventing the environmental impact of food waste for the specific context of the case study. According to the results obtained, for each kg of lettuce that is not produced, 0.065 kg CO2eq are avoided. These findings also highlighted the environmental efficacy of animal feed production, which is a cost-efficient strategy, widely prevalent in the city studied, presents a carbon footprint of -0.013 kg CO2eq/kg of waste. Other common solutions implemented at the case study, such as anaerobic digestion and composting exhibit less favourable carbon footprints, measuring 0.019 and 0.006 kg CO2eq/kg of lettuce, respectively. Landfilling emerges, as expected, as the least desirable option with a considerably higher carbon footprint of 0.423 kg CO2eq/kg. To summarise, this study highlights the environmental advantages of prioritising prevention and higher waste hierarchy levels. It underscores the need for context-specific evaluations when dealing with the intricacies of waste management systems. Moreover, the research emphasises the potential for innovative strategies, multi-stakeholder collaboration, and a holistic approach to address the complex issue of FLW, considering both the environmental impact and practical challenges in a real-world implementation.