Dependence on oil imported from foreign countries affect the National Energy securities and Energy security of global economies has become one of the most challenging problem that needs to be resolved as the fossil sources are fast diminishing and irreplaceable. The alarming energy demand and consumption rate of the present global status is currently exponentially exceeding the rate of local supply sources, becoming an issue of concern. A look beyond the fossils is crucial for long tern economic growth and energy security as there are numerous uncertainties about the fossil supplies coupled with the greater environmental risks encountered during exploitation. Thus the new concept for treating ethanol distillery waste anaerobically to produce Biogas- a clean renewable alternative energy with many applications projects sustainable and more realistic option. The research project focuses on Comparison of the Potentials and Efficacy of Anaerobic Digestion of Stillage (Wheat Stillage and Lignocellulose Stillage) from Ethanol Distillery plants for Biogas Production. It also investigates better alternative temperature dependent Stillage Anaerobic Digestion that will enhance a higher Biogas yield. Anaerobic digestions were performed in triplicate batch systems, during both mesophilic (35 °C) and thermophilic (55 °C) conditions at a period of 50-days. The reactors contained 2.73g of Wheat Stillage and 5.2g of Lignocellulose Stillage samples, respectively, corresponding to 2% VS in each reactor. The inoculum was taken from either a mesophilic Biogas Plant (Gässlosa., Borås), or from a thermophilic Biogas Plant (Sobacken, Borås). A Gas Chromatographic method (GC) was employed for determination of the obtained biogas composition. The theoretical CH4 Potential for Wheat Stillage and Lignocellulose Stillage is 0.473 m3CH4/kg VS and 0.407 m3CH4/kg VS, respectively. The results obtained from this study indicated, however, that the Wheat Stillage performed better under thermophilic conditions with a peak of 575ml CH4 / 0.5g VS; while the Lignocellulosic Stillage gave the best performance under mesophilic conditions leading to a methane production of 436ml CH4/ 0.5g VS after 4 weeks of digestion period.