Based on the sustainable development practice-zero growth in chemical fertilizer application, this article used bagasse organic fertilizer and rice husk derived biochar to investigate the response of soil bacterial community in apple orchard. Aimed at realize the soil quality improvement and biomass resource recovery to contribute agricultural and environmental sustainability. The co-trophic Proteobacteria was predominant in all the treatments (29–36 %) and enriched in non-nitrifying Alphaproteobacteria (9–11 %) and ammonia oxidant Betaproteobacteria (8–10 %), especially richest in bagasse fertilizer combine biochar treated soil. In addition, bacterial community variation was assessed by alpha and beta diversity, four treatments dispersed distribution and richer abundance observed in combined apply bagasse fertilizer and biochar treatment (3909.22 observed-species) than single application (3729.88 and 3646.58 observed-species). Biochar as microbial carrier combined organic fertilizer were established synergistic interaction and favorable to organic matter availability during sustainable agriculture. Finally, integrated biochar-bagasse fertilizer was richer than single organic or biochar fertilization in improving soil bacterial diversity, notably by promoting the metabolism of copiotrophic bacteria, nutrient cycling, plant growth and disease inhibit-related bacteria.