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  • 1.
    Kahoush, May
    University of Borås, Faculty of Textiles, Engineering and Business. ENSAIT.
    Bio-functionalization of conductive textile materials with redox enzymes2017In: IOP Conference Series: Materials Science and Engineering, 2017, Vol. 254, article id 112002Conference paper (Refereed)
    Abstract [en]

    In recent years, immobilization of oxidoreductase enzymes on electrically conductive materials has played an important role in the development of sustainable bio-technologies. Immobilization process allows the re-use of these bio-catalysts in their final applications.

    In this study, different methods of immobilizing redox enzymes on conductive textile materials were used to produce bio-functionalized electrodes. These electrodes can be used for bio-processes and bio-sensing in eco-designed applications in domains such as medicine and pollution control.

    However, the main challenge facing the stability and durability of these electrodes is the maintenance of the enzymatic activity after the immobilization. Hence, preventing the enzyme’s denaturation and leaching is a critical factor for the success of the immobilization processes. 

  • 2.
    Kahoush, May
    University of Borås, Faculty of Textiles, Engineering and Business.
    Eco-Technologies for Immobilizing Redox Enzymes on Conductive Textiles, for Sustainable Development2019Doctoral thesis, monograph (Other academic)
    Abstract [en]

    Enzyme immobilization on electrically conductive supports is necessary to improve their bioactivity and stability, for use and re-use in applications depending on bio-electrochemical response, such as in bioelectrodes, biosensors, or biofuel cells. However, the immobilization methods used are still facing many challenges in terms of health hazards and high environmental impact. Thus, it is important to find balanced and eco-friendly approaches to achieve efficient immobilization with minimum harmful consequences.

    Hence, within the frame of this thesis, the use of eco-technologies such as cold remote plasma, a bio-compatible conductive (PEDOT:PSS) polymer coating, and a bio-based crosslinker “genipin” which has low toxicity, to immobilize glucose oxidase (GOx) enzyme on conductive carbon fiber-based nonwoven textiles was investigated. These carbon-based textiles, regardless of their hydrophobicity, are robust materials to be used as alternative for expensive rigid metals, since they possess good electrical conductivity and good resistance to corrosion in different media.

    The results obtained showed that cold remote plasma treatment with nitrogen and oxygen gas mixture was efficient in functionalizing the surface of carbon felts and PEDOT:PSS coated felts. This increased carbon fiber surface energies, and facilitated the immobilization of GOx by physical adsorption with maintained bioactivity and improved reusability. Furthermore, immobilization of GOx using genipin as a crosslinking agent improved remarkably the stability of performance of bio-functionalized carbon felts. This crosslinker showed to be able to directly crosslink the enzymes without a matrix or hydrogel. Finally, the obtained bio-functionalized carbon textiles were primarily evaluated for use in sustainable applications for wastewater treatment such as Bio-Fenton (BF) and enzymatic Bio-Electro-Fenton (BEF). The results showed that bioactivity and bio-electro-activity of immobilized GOx was promising in color removal of Remazol Blue RR reactive dye and its partial degradation from solution in both treatments, which proved the success of the chosen immobilization methods in producing bioactive textiles that can be used as electrodes for power generation and pollution control.

  • 3.
    Kahoush, May
    et al.
    University of Borås, Faculty of Textiles, Engineering and Business. ENSAIT.
    Behary, Nemeshwaree
    Université de Lille, Nord de France, France.
    Aurélie, Cayla
    Université de Lille, Nord de France, France.
    Brigitte, Mutel
    Université de Lille, Nord de France, France.
    Jinping, Guan
    College of Textile and Clothing Engineering, Soochow University, Suzhou, China.
    Nierstrasz, Vincent
    University of Borås, Faculty of Textiles, Engineering and Business.
    Surface modification of carbon felt by cold remote plasma for glucose oxidase enzyme immobilization2019In: Applied Surface Science, ISSN 0169-4332, E-ISSN 1873-5584, Vol. 467, p. 1016-1024Article in journal (Refereed)
    Abstract [en]

    Despite their chemical inertness and poor hydrophobicity, carbon-based materials are widely used in electrochemical applications due to their robustness, good electrical conductivity and corrosion resistance. The purpose of the work carried was to increase the wettability of nonwoven carbon fiber felts for improved efficiency in bio/electrochemical applications. Virgin Carbon Felt (VCF) was first treated with cold remote plasma (CRP) using a mixture of nitrogen and oxygen (1 or 2 %) as plasma gas.  Bio-functionalization of the carbon felts with glucose oxidase (GOx) enzyme was then carried using physical adsorption method. FTIR and XPS analysis showed an integration of new oxygenated functional groups (C-O and C=O) as well as amines and amides on the surface of VCF treated by the CRP treatment, which improved the wettability of the samples. Capillary uptake increased from around 0 % (for VCF) to nearly 750 % with 2 % oxygen in plasma gas. GOx enzyme showed higher activity after immobilization at pH 5.5 on the CRP treated samples, maintaining up to 50 % of its initial enzymatic activity after six cycles while with the VCF, no enzymatic activity was observed after the fourth cycle. These obtained felts can be used as electrodes in sustainable bioprocesses.

  • 4.
    Kahoush, May
    et al.
    University of Borås, Faculty of Textiles, Engineering and Business.
    Behary, Nemeshwaree
    ENSAIT .
    Cayla, Aurélie
    ENSAIT.
    Guan, Jinping
    Soochow University .
    Nierstrasz, Vincent
    University of Borås, Faculty of Textiles, Engineering and Business.
    Bio-Electro-Fenton for the Treatment of Textile Wastewater2018In: Proceedings of the 10th International Conference of Fiber and Polymer Biotechnology / [ed] Jürgen Andreaus, 2018, Vol. 1, p. 73-74, article id OP 22Conference paper (Refereed)
  • 5.
    Kahoush, May
    et al.
    University of Borås, Faculty of Textiles, Engineering and Business. ENSAIT.
    Behary, Nemeshwaree
    ENSAIT.
    Cayla, Aurélie
    ENSAIT.
    Nierstrasz, Vincent
    University of Borås, Faculty of Textiles, Engineering and Business.
    Bio-Fenton and Bio-Electro-Fenton as sustainable methods for degrading organic pollutants in wastewater2018In: Process Biochemistry, ISSN 1359-5113, E-ISSN 1873-3298, Vol. 64C, p. 237-247Article in journal (Refereed)
    Abstract [en]

    In this paper, an overview of the bio-Fenton and bio-electro-Fenton processes for sustainable wastewater treatment is provided. These two methods have been used in recent years to treat many kinds of persistent pollutants while maintaining the sustainability in materials and power consumption compared to conventional methods, through efficient eco-designed systems. The different kinds of electrodes used for the bio-electro-Fenton are reviewed, along with the influencing factors affecting the efficiency of these methods, and the different designs used to construct the reactors. Moreover, the various organic pollutants from industrial sources, like effluents from textile and pesticides facilities, treated using these processes are also reported. However, the main challenge facing these technologies is to improve their performance, stability and lifetime to achieve more sustainable and cost-effective wastewater treatment on pilot and large scales. Hence, future perspectives and trends are discussed to overcome the drawbacks of these methods.

  • 6.
    Kahoush, May
    et al.
    University of Borås, Faculty of Textiles, Engineering and Business.
    Behary, NemshwareeENSAIT.Cayla, AurileENSAIT.
    Comparison of three different methods for immobilizing redox enzymes on a multi-filament conductive carbon yarn2016Conference proceedings (editor) (Refereed)
1 - 6 of 6
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