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  • 1.
    Lukitawesa, Lukitawesa
    et al.
    University of Borås, Faculty of Textiles, Engineering and Business.
    Wikandari, R.
    Department of Food and Agricultural Product Technology, Universitas Gadjah Mada.
    Millati, R.
    Department of Food and Agricultural Product Technology, Universitas Gadjah Mada.
    Taherzadeh, Mohammad J
    University of Borås, Faculty of Textiles, Engineering and Business.
    Niklasson, C.
    Department of Chemical Reaction Engineering, Chalmers University of Technology.
    Effect of effluent recirculation on biogas production using two-stage anaerobic digestion of citrus waste2018In: Molecules, ISSN 1420-3049, E-ISSN 1420-3049, Vol. 23, no 12, article id 3380Article in journal (Refereed)
    Abstract [en]

    Citrus waste is a promising potential feedstock for anaerobic digestion, yet the presence of inhibitors such as d-limonene is known to limit the process. Effluent recirculation has been proven to increase methane yield in a semi-continuous process for recalcitrant material, but it has never been applied to toxic materials. This study was aimed to investigate the effect of recirculation on biogas production from citrus waste as toxic feedstock in two-stage anaerobic digestion. The first digestion was carried out in a stirred tank reactor (STR). The effluent from the first-stage was filtered using a rotary drum filter to separate the solid and the liquid phase. The solid phase, rich in hydrophobic D-limonene, was discarded, and the liquid phase containing less D-limonene was fed into the second digester in an up-flow anaerobic sludge bed (UASB) reactor. A high organic loading rate (OLR 5 g VS/(L·day)) of citrus waste was fed into the first-stage reactor every day. The effluent of the first-stage was then fed into the second-stage reactor. This experiment was run for 120 days. A reactor configuration without recirculation was used as control. The result shows that the reactor with effluent recirculation produced a higher methane yield (160–203 NmL/g·VS) compared to that without recirculation (66–113 NmL/g·VS). More stable performance was also observed in the reactor with recirculation as shown by the pH of 5–6, while without recirculation the pH dropped to the range of 3.7–4.7. The VS reduction for the reactor with recirculation was 33–35% higher than that of the control without recirculation. Recirculation might affect the hydrolysis-acidogenesis process by regulating pH in the first-stage and removing most of the D-limonene content from the substrate through filtration. 

  • 2.
    Shahryari, Zohre
    et al.
    Department of Chemical Engineering, Faculty of Engineering, Shahid Bahonar University of Kerman.
    Fazaelipoor, Mohammad H.
    Department of Chemical Engineering, Faculty of Engineering, Shahid Bahonar University of Kerman.
    Ghasemi, Younes
    Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences.
    Lennartsson, Patrik R.
    University of Borås, Faculty of Textiles, Engineering and Business.
    Taherzadeh, Mohammad J
    University of Borås, Faculty of Textiles, Engineering and Business.
    Amylase and Xylanase from Edible Fungus Neurospora intermedia: Production and Characterization2019In: Molecules, ISSN 1420-3049, E-ISSN 1420-3049, Vol. 24, no 4Article in journal (Refereed)
    Abstract [en]

    Integrated enzyme production in the biorefinery can significantly reduce the cost of the entire process. The purpose of the present study is to evaluate the production of two hydrolyzing enzymes (amylase and xylanase) by an edible fungus used in the biorefinery, Neurospora intermedia. The enzyme production was explored through submerged fermentation of synthetic media and a wheat-based waste stream (thin stillage and wheat bran). The influence of a nitrogen source on N. intermedia was investigated and a combination of NaNO3 and yeast extract has been identified as the best nitrogen source for extracellular enzyme production. N. intermedia enzymes showed maximum activity at 65 degrees C and pH around 5. Under these conditions, the maximum velocity of amylase and xylanase for starch and xylan hydrolysis was found to be 3.25 U mL(-1) and 14.77 U mL(-1), respectively. Cultivation of N. intermedia in thin stillage and wheat bran medium resulted in relatively high amylase (8.86 +/- 0.41 U mL(-1), 4.68 +/- 0.23) and xylanase (5.48 +/- 0.21, 2.58 +/- 0.07 U mL(-1)) production, respectively, which makes this fungus promising for enzyme production through a wheat-based biorefinery.

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