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  • 1.
    Biswas, Tuser
    et al.
    University of Borås, Faculty of Textiles, Engineering and Business.
    Yu, Junchun
    University of Borås, Faculty of Textiles, Engineering and Business.
    Nierstrasz, Vincent
    University of Borås, Faculty of Textiles, Engineering and Business.
    Effective Pretreatment Routes of Polyethylene Terephthalate Fabric for Digital Inkjet Printing of Enzyme2021In: Materials Science & Engineering: B. Solid-state Materials for Advanced Technology, ISSN 0921-5107, E-ISSN 1873-4944Article in journal (Refereed)
    Abstract [en]

    Enzymes immobilized on synthetic polyethylene terephthalate (PET) textile surface by resource‐efficient inkjet printing technology can promote developments for various novel applications. Synthetic fabrics often require adequate pretreatments to facilitate such printing process. This work discusses PET–woven fabric pretreatment routes to improve wettability by alkaline, enzymatic, and plasma processes for effective printing of lysozyme using an industrial piezoelectric printhead. Results indicate that all pretreated samples contain a similar amount of enzymes upon printing. Plasma treated fabrics show relatively more hydrophilic surface characteristics, better protein binding stability, and lower retained activity. Alkali and cutinase‐treated samples possess relatively higher activity due to the greater amount of enzyme desorption to substrate solution. Depending on respective enzyme‐binding stability, a combination of a well-pretreated surface and inkjet as preferential placement technology, the approach of this study can be used as a facile enzyme immobilization method for suitable applications, for example, controlled‐release and bio‐sensing.

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  • 2.
    Ruan, Yunjie
    et al.
    Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems, Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
    Mukesh Kumar, Awasthi
    University of Borås, Faculty of Textiles, Engineering and Business. College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, China.
    Cai, Lei
    Laboratory of Microbial Resources, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China.
    Lu, Huifeng
    Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
    Xu, Xiangyang
    Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
    Li, Wenbing
    Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
    Simultaneous aerobic denitrification and antibiotics degradation by strain Marinobacter hydrocarbonoclasticus RAD-22020In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 313Article in journal (Refereed)
    Abstract [en]

    Simultaneous denitrification and antibiotics (oxytetracycline, OTC and ciprofloxacin, CFX) degradation was evaluated using a typical aerobic denitrifying strain Marinobacter hydrocarbonoclasticus RAD-2. There was no significant influence on the aerobic nitrate removal efficiency of strain RAD-2 in the presence of these two antibiotics. Along with denitrification, the average degradation rate of 2.92 mu g OTC L(-1)h(-1) was achieved, while no degradation was observed for CFX. The growth behavior indicated that an insignificant inhibition effect could have occurred at an antibiotics dosage lower than 300 mu g/L. The transcriptional results revealed that antibiotics exposure caused (< 2h) down-regulation of the denitrifying related genes, but triggered a significant subsequent up-regulation (4 h). Less nitrous oxide productions were observed in both aerobic and anoxic denitrification processes with antibiotics. Overall, the hormesis effect caused by antibiotics exposure indicated a potential approach to enhance the co-metabolism degradation performance for nitrate and antibiotics in aerobic denitrification.

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