A novel method of lignocellulosic ethanol production was developed and evaluated, “Simultaneous Saccharification Filtration and Fermentation (SSFF)”. SSFF is an integrated process which combines the advantages of both Separate hydrolysis and fermentation (SHF) and Simultaneous saccharification and fermentation (SSF). The process involves simultaneous enzymatic hydrolysis of lignocellulosic biomass, filtration and fermentation of the filtrate with yeast Saccharomyces cerevisiae as fermenting organism. Different suspended solid (SS) were evaluated to determine what solid concentration can be pumped through the filtration device and the life span of a cross-flow filter module was assessed. Capacity tests were performed on the fermentation unit to determine the uptake capability of the fermenting organism. It was furthermore investigated how long the cells can be successfully reused. It was observed that up to 14% solids concentration could be pumped through the filtration unit. After enzymatic treatment, a slurry with 14.4% initial SS was filtered continuously for 28 days without clogging or fouling. A flocculating yeast strain (CCUG 53310) was able to consume the glucose from the hydrolysis through the filtration effectively and the yeast culture was reused for 5 batches of SSFF. The SSFF cultivations resulted in an ethanol yield of up to 85.0% of the theoretical yield. Our new process of SSFF could potentially be used in lignocellulosic ethanol production.