Empty fruit bunches (EFB) is a lignocellulosic residue after palm oil extraction and can be a cheap feedstock for lignocellulosic ethanol production. EFB was pretreated with phosphoric acid as a means of chemical pretreatment and white rot fungi Pleurotus floridanus LIPIMC966 as a means of biological pretreatment, both methods were later combined. The pretreated materials were fermented to ethanol with simultaneous saccharification and fermentation (SSF). SSF was performed at temperature of 31°C and a pH of 5.0. Cellulase enzyme Cellic® CTec2, was used for the hydrolysis and yeast Saccharomyces cerevisiae CBS 8066 was used for the fermentation. Hydrolysis alone was also carried out on the EFB so as to measure digestibility with the different pretreatments. Pretreatment with combination of the two methods, phosphoric acid pretreatment and fungal pretreatment improves the digestibility by 7.4, 6.3 and 4.0 folds respectively. During the SSF, phosphoric acid pretreatment gave the highest ethanol yield of 77.2% of the theoretical yield at 48 h, combination of phosphoric and fungal pretreatment gave highest yeild of 76% at 48 h while a yield of 24% was produced as the highest yeild from the fungal pretreated material at 72 h. This study shows that a combination of phosphoric acid and fungal pretreatment could potentially increase the ethanol yield from this lignocellulosic material.