Seven zygomycetes tempe isolates were screened for ethanol production at 40oC, 45oC, 50oC. All the isolates were able to produce ethanol at 40oC and three isolates were able to produce ethanol at 45oC. The corresponding ethanol yields were 0.39-0.47 g/g on glucose and 0.22- 0.26 g/g on glucose. No isolate could grow and produce ethanol at 50oC. One isolate was selected and used for ethanol production from untreated and fungal-pretreated oil palm empty fruit bunch (OPEFB), untreated and fungal-pretreated oat straw as well as Avicel by nonisothermal simultaneous saccharification and fermentation (NSSF). This NSSF included hydrolysis with cellulase and -glucosidase for 24 h at 45oC, followed by simultaneous saccharification and fermentation with the selected isolate at 40oC for 96 h. The NSSF of untreated and fungal-pretreated OPEFB, untreated and fungal-pretreated oat straw, and Avicel resulted in 14.10%, 14.25%, 27.28%, 49.88%, and 63.77% ethanol compared to the theoretical yield.