Two different wool based textile wastes (TW1 and TW2) have been subjected for biogas production. TW1 was composed of 70% wool and 30% polyamide (PA), while TW2 consisted of 70% wool, 18% PA and 12% kermel (protective polyamide-imide fibre). Two pre-treatments: thermal treatment, enzymatic treatment and combinations of these two were performed to enhance the methane yield. Determining the soluble protein concentrations in the treated samples showed that the additional thermal treatment and the enzyme concentration had significant positive effect on the degradation of wool. Samples treated with thermal and combination treatments were therefore selected for anaerobic batch digestion assays. The best results were obtained after combination treatments resulting in methane yields of 0.33-0.43 Nm3/kg VS, and 0.21-0.26 Nm3/kg VS, for TW1 and TW2, respectively, while only 0.21 and 0.05 Nm3/kg VS methane production was measured after the thermal treatment. The methane yields of untreated samples were close to zero.