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Inhibitor tolerance and flocculation: Characterization of a yeast strain suitable for 2nd generation bioethanol production
University of Borås, School of Engineering.
University of Borås, School of Engineering.ORCID iD: 0000-0003-4887-2433
2011 (English)Conference paper, Poster (with or without abstract) (Other academic)
Sustainable development
The content falls within the scope of Sustainable Development
Abstract [en]

Robust second generation bioethanol processes require microorganisms able to obtain high yields and production rates while fermenting inhibiting hydrolysates. However, tolerance towards inhibitors like, carboxylic acids, furan aldehydes and phenolic compounds, is still an issue and the factors contributing to improved tolerance are not well known. In this study, the constitutively flocculating Saccharomyces cerevisiae strain CCUG 53310, with good ability to ferment toxic hydrolysates, was compared with S. cerevisiae CBS 8066 in order to characterize the mechanisms of flocculation and the fermentative performance in different inhibitory media. The flocculation of CCUG 53310 depended on cell wall proteins and was partly inhibited by mannose. The flocculating cells also exhibited a significantly higher hydrophobicity than the cells of the non-flocculating strain CBS 8066, which might contribute to the flocculation. The flocculating strain was more tolerant to carboxylic acids and furan aldehydes, but more sensitive to phenolic compounds. Surprisingly, the expression increase of YAP1, ATR1 and FLR1, known to confer resistance against lignocellulose-derived inhibitors, upon addition of various inhibitors to the fermentation medium, was less in CCUG 53310 than in CBS 8066 in most cases. This indicates that the flocculating strain experienced the cultivation conditions as less stressful. The flocculation in itself is a likely cause of this by creating subinhibitory local levels of inhibitors for most cells, allowing the cells in flocs to experience a lower collective stress level.

Place, publisher, year, edition, pages
2011.
Keywords [en]
yeast, saccharomyces, bioethanol, biofuel, liquid core encapsulation, gene expression, YAP1, ATR1, FLR1
Keywords [sv]
Energi och material
National Category
Microbiology Other Industrial Biotechnology
Research subject
Resource Recovery
Identifiers
URN: urn:nbn:se:hb:diva-6765Local ID: 2320/10322OAI: oai:DiVA.org:hb-6765DiVA, id: diva2:887467
Conference
Joint poster exhibition at the Dept Chemical and Biological Engineering,Chalmers University of Technology, and Dept Chemistry, University of Gothenburg. April 12, 2011
Available from: 2015-12-22 Created: 2015-12-22 Last updated: 2017-01-26Bibliographically approved

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Westman, Johan O.Taherzadeh, Mohammad J

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CiteExportLink to record
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