The ability of macroencapsulated Saccharomyces cerevisiae CBS 8066 to produce previous termethanolnext term at previous termelevatednext termprevious termtemperaturesnext term was investigated in consecutive batch and continuous cultures. Prior to cultivation previous termyeastnext term was confined inside alginate–chitosan capsules composed of an outer semi-permeable membrane and an inner liquid core. The encapsulated previous termyeastnext term could successfully ferment 30 g/L glucose and produce previous termethanolnext term at a high yield in five consecutive batches of 12 h duration at 42 °C, while freely suspended previous termyeastnext term was completely inactive already in the third batch. A high previous termethanolnext termprevious termproductionnext term was observed also through the first 48 h at 40 °C during continuous cultivation at D = 0.2 h−1 when using encapsulated cells. The previous termethanolnext termprevious termproductionnext term slowly decreased in the following days at 40 °C. The previous termethanolnext termprevious termproductionnext term was also measured in a continuous cultivation in which the previous termtemperaturenext term was periodically increased to 42–45 °C and lowered to 37 °C again in periods of 12 h. Our investigation shows that a non-thermotolerant previous termyeastnext term strain improved its heat tolerance upon previous termencapsulationnext term, and could produce previous termethanolnext term at previous termtemperaturesnext term as high as 45 °C for a short time. The possibility of performing fermentations at higher previous termtemperaturesnext term would greatly improve the enzymatic hydrolysis in simultaneous saccharification and fermentation (SSF) processes and thereby make the bioethanol previous termproductionnext term process more economically feasible.