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RNA silencing of lactate dehydrogenase gene in Rhizopus oryzae
University of Borås, School of Engineering.
University of Borås, School of Engineering.ORCID iD: 0000-0003-4887-2433
2011 (English)In: Journal of RNAi and Gene Silencing, ISSN 1747-0854, E-ISSN 1747-0854, ISSN 1747-0854, Vol. 7, 443-448 p.Article in journal (Refereed) Published
Abstract [en]

Rhizopus oryzae is a filamentous fungus, belonging to the order Mucorales. It can ferment a wide range of carbohydrates hydrolyzed from lignocellulosic materials and even cellobiose to produce ethanol. However, R. oryzae also produces lactic acid as a major metabolite, which reduces the yield of ethanol. In this study, we show that significant reduction of lactic acid production could be achieved by short (25nt) synthetic siRNAs targeting the ldhA gene. The average yield of lactic acid production by R. oryzae during the batch fermentation process, where glucose had been used as a sole carbon source, diminished from 0.07gm/gm in wild type to 0.01gm/gm in silenced samples. In contrast, the average yield of ethanol production increased from 0.39gm/gm in wild type to 0.45gm/gm in silenced samples. These results show 85.7% (gm/gm) reduction in lactic acid production as compared with the wild type R. oryzae, while an increase of 15.4% (gm/gm) in ethanol yield.

Place, publisher, year, edition, pages
Library Publishing Media , 2011. Vol. 7, 443-448 p.
Keyword [en]
delivery, rhizopus oryzae, ldha gene, lactate dehydrogenase, lactic acid, ethanol production
Keyword [sv]
sirna, Energi och material
National Category
Environmental Biotechnology
Identifiers
URN: urn:nbn:se:hb:diva-3138Local ID: 2320/8524OAI: oai:DiVA.org:hb-3138DiVA: diva2:871234
Available from: 2015-11-13 Created: 2015-11-13 Last updated: 2017-08-23Bibliographically approved

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CiteExportLink to record
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