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Pretreatment of spruce and oak by N-methylmorpholine-N-oxide (NMMO) for efficient conversion of their cellulose to ethanol
University of Borås, School of Engineering.ORCID iD: 0000-0003-4887-2433
2010 (English)In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 101, no 13, 4914-4918 p.Article in journal (Refereed) Published
Abstract [en]

Pretreatment of softwood spruce and hardwood oak with an industrial cellulose solvent, N-methylmorpholine-N-oxide (NMMO), was investigated prior to enzymatic hydrolysis and fermentation to ethanol. The pretreatments were carried out at 90, 110 and 130 °C for 1–3 h with 85% NMMO solution, followed by non-isothermal simultaneous saccharification and fermentation (NSSF). This NSSF included hydrolysis with cellulase and β-glucosidase for 24 h at 45 °C, followed by continuous saccharification and fermentation with Saccharomyces cerevisiae at 37 °C for 3 days. The NSSF of untreated oak and spruce resulted in 18.6% and 6.8% ethanol compared to the maximum theoretical yield. However, the pretreatment of oak and spruce at 130 °C resulted in almost total conversion of cellulose to ethanol and improved ethanol yield up to 85.4% and 89%, respectively. These numbers are comparable with ethanol from pure glucose with the same strain, which yielded between 84% and 90% of the theoretical ethanol yield.

Place, publisher, year, edition, pages
Elsevier BV , 2010. Vol. 101, no 13, 4914-4918 p.
Keyword [sv]
Energi och material
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:hb:diva-2856DOI: 10.1016/j.biortech.2009.08.100Local ID: 2320/6949OAI: oai:DiVA.org:hb-2856DiVA: diva2:870950
Available from: 2015-11-13 Created: 2015-11-13 Last updated: 2016-09-29Bibliographically approved

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