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Effect of bioactive extruded PLA/HA composite films on focal adhesion formation of preosteoblastic cells
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2014 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 121, p. 409-416Article in journal (Refereed) Published
Abstract [en]

The quality of the initial cell attachment to a biomaterial will influence any further cell function, including spreading, proliferation, differentiation and viability. Cell attachment is influenced by the material's ability to adsorb proteins, which is related to the surface chemistry and topography of the material. In this study, we incorporated hydroxyapatite (HA) particles into a poly(lactic acid) (PLA) composite and evaluated the surface structure and the effects of HA density on the initial cell attachment in vitro of murine calvarial preosteoblasts (MC3T3-EI). Scanning electron microscopy (SEM), atomic force microscopy (AFM) and infrared spectroscopy (FTIR) showed that the HA particles were successfully incorporated into the PLA matrix and located at the surface which is of importance in order to maintain the bioactive effect of the HA particles. SEM and AFM investigation revealed that the HA density (particles/area) as well as surface roughness increased with HA loading concentration (i.e. 5, 10, 15 and 20wt%), which promoted protein adsorption. Furthermore, the presence of HA on the surface enhanced cell spreading, increased the formation of actin stress fibers and significantly improved the expression of vinculin in MC3T3-E1 cells which is a key player in the regulation of cell adhesion. These results suggest the potential utility of PLA/HA composites as biomaterials for use as a bone substitute material and in tissue engineering applications.

Place, publisher, year, edition, pages
Elsevier BV , 2014. Vol. 121, p. 409-416
Keywords [en]
poly lactic acid, melt extrusion, cell adhesion, Resursåtervinning
National Category
Textile, Rubber and Polymeric Materials
Research subject
Resource Recovery
Identifiers
URN: urn:nbn:se:hb:diva-1945DOI: 10.1016/j.colsurfb.2014.06.029ISI: 000341335800050PubMedID: 24986753Local ID: 2320/14404OAI: oai:DiVA.org:hb-1945DiVA, id: diva2:870023
Available from: 2015-11-13 Created: 2015-11-13 Last updated: 2017-10-12Bibliographically approved

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Skrifvars, Mikael

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