Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • harvard-cite-them-right
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A Bacillus Strain Able to Hydrolyze Alpha- and Beta-Keratin
University of Borås, School of Engineering. (Biotechnology)
University of Borås, School of Engineering. (Biotechnology)
University of Borås, School of Engineering. (Biotechnology)ORCID iD: 0000-0003-4887-2433
2014 (English)In: Journal of Bioprocessing & Biotechniques, ISSN 2155-9821, Vol. 4, p. 181-Article in journal (Refereed)
Sustainable development
The content falls within the scope of Sustainable Development
Abstract [en]

The ability to hydrolyze keratin, a rigid and strongly cross-linked protein in the waste of poultry feather and sheep wool, has made keratinase production by microorganisms highly important to the biotechnological industry. A proteindegrading bacterium (C4) was isolated from compost. Based on morphology and biochemical tests, along with 16S rRNA sequencing, the isolated C4 was tentatively identified as Bacillus sp. C4 (2008). The proteolytic activity of the Bacillus sp. C4 strain was broadly specific; it degraded keratinous and non-keratinous proteins to different degrees. Pea pods as substrate generated the highest protease production, followed by soybean meal and sheep wool. Notwithstanding, using wool keratin as a sole source of carbon and nitrogen yielded the highest level of soluble proteins. Furthermore, the C4 bacterium grew well, and produced a significant level of keratinase when using wool and feather as substrates. Supplementing the medium with yeast extract and peptone shortened the time required for feather degradation, but delayed the onset of the wool keratin hydrolysis with two days. The predominant amino acids released in feather hydrolysate were tyrosine, phenylalanine, and histidine. In contrast, the wool lysate was rich in aspartic acid, methionine, tyrosine, phenylalanine, histidine, and lysine. Results established that utilizing the C4 strain for keratin degradation in waste management holds considerable potential.

Place, publisher, year, edition, pages
Omics Publishing Group , 2014. Vol. 4, p. 181-
Keywords [en]
Resource Recovery
National Category
Industrial Biotechnology
Research subject
Resource Recovery
Identifiers
URN: urn:nbn:se:hb:diva-1938DOI: 10.4172/2155-9821.1000181Local ID: 2320/14387OAI: oai:DiVA.org:hb-1938DiVA, id: diva2:870016
Available from: 2015-11-13 Created: 2015-11-13 Last updated: 2016-03-03

Open Access in DiVA

fulltext(1994 kB)872 downloads
File information
File name FULLTEXT01.pdfFile size 1994 kBChecksum SHA-512
d39d0d17cee4bc08c6edfc9bc36a60564c82f14276f0e2d62f1fd01c540c565f2e2dea4bce48e2775eae02b985463e9d385f7ca2c031f35386b92a5e42ee5475
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records

Fellahi, SoltanaFeuk-Lagerstedt, ElisabethTaherzadeh, Mohammad J.

Search in DiVA

By author/editor
Fellahi, SoltanaFeuk-Lagerstedt, ElisabethTaherzadeh, Mohammad J.
By organisation
School of Engineering
Industrial Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
Total: 872 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 1289 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • harvard-cite-them-right
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf