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Pellet formation of zygomycetes and immobilization of yeast
University of Borås, School of Engineering.
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2013 (English)In: New Biotechnology, ISSN 1871-6784, E-ISSN 1876-4347, Vol. 30, no 5, p. 516-522Article in journal (Refereed) Published
Sustainable development
The content falls within the scope of Sustainable Development
Abstract [en]

Pelleted growth provides many advantages for filamentous fungi, including decreased broth viscosity, improved aeration, stirring, and heat transfer. Thus, the factors influencing the probability of pellet formation of Rhizopus sp. in a defined medium was investigated using a multifactorial experimental design. Temperature, agitation intensity, Ca2+-concentration, pH, and solid cellulose particles, each had a significant effect on pelletization. Tween 80, spore concentration, and liquid volume were not found to have a significant effect. All of the effects were additive; no interactions were significant. The results were used to create a simple defined medium inducing pelletization, which was used for immobilization of a flocculating strain of Saccharomyces cerevisiae in the zygomycetes pellets. A flor-forming S. cerevisiae strain was also immobilized, while a non-flocculating strain colonized the pellets but was not immobilized. No adverse effects were detected as a result of the close proximity between the filamentous fungus and the yeast, which potentially allows for co-fermentation with S. cerevisiae immobilized in pellets of zygomycetes

Place, publisher, year, edition, pages
Elsevier BV , 2013. Vol. 30, no 5, p. 516-522
Keywords [en]
Fungal Pellets, Immobilization, Zygomycetes, Rhizopus, Saccharomyces cerevisiae, flocculation, Resursåtervinning
National Category
Engineering and Technology Industrial Biotechnology
Research subject
Resource Recovery
Identifiers
URN: urn:nbn:se:hb:diva-1699DOI: 10.1016/j.nbt.2013.05.007ISI: 000321695200012PubMedID: 23711366Local ID: 2320/13098OAI: oai:DiVA.org:hb-1699DiVA, id: diva2:869768
Available from: 2015-11-13 Created: 2015-11-13 Last updated: 2017-12-01Bibliographically approved

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Westman, JohanLundin, MagnusLennartsson, PatrikTaherzadeh, Mohammad

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