The combination of utilisation of fibres from industrial hemp waste and degumming using deep eutectic solvents can contribute to a more sustainable textile industry. Degumming of hemp is necessary to remove gummy matters such as lignin and hemicellulose since they bind the fibres together into bundles and hinder their use in textile applications. This study therefore evaluates the effect of deep eutectic solvent treatment on fibres from Finola hemp grown for food production. After a pre-study, a deep eutectic solvent consisting of choline chloride and urea with molar ratio 1:2 was chosen as well as a microwave assisted treatment method. The number of microwave heating cycles as well as the bath ratio was further evaluated through a full factorial design with two levels. The physical, mechanical, chemical, and thermal effects from the treatment on the fibres were evaluated through a series of tests. The results were compared to three references: untreated Finola hemp, alkali treated Finola hemp, and deep eutectic solvent treated fibre hemp. The results showed that the treatment had a degumming effect on the fibres. It was indicated by for example significant weight reduction, smaller diameter, decrease in initial modulus, and removal of the peak linked to hemicellulose in DTG for the treated fibres. It was also confirmed by the chemical composition testing that showed lower lignin and hemicellulose content for the treated fibres. After evaluation, the most efficient treatment combination was concluded to be treatment at 100 W/g with bath ratio 1:20 (weight:volume) and 12 heating cycles consisting of 0.5 min microwave heating with 1 min between. The treatment had the smallest diameter, highest weight reduction, and most visible changes in the FTIR spectrum. The fibres did have a significant reduction in mean length, from 37.8 mm to 29.8 mm. The other results were however deemed to outweigh the length reduction. That treatment combination was therefore used for evaluation of the reusability of the deep eutectic solvent. The same solvent was used for a total of 3 treatment cycles and the effect on the fibre properties were evaluated. No statistically significant changes in fibre properties were caused by the reuse, which further increases the sustainability advantages with deep eutectic solvent degumming compared to traditional alkali degumming.