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Boosting enzymatic degradation of cellulose using a fungal expansin: Structural insight into the pretreatment mechanism
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Department of Chemistry, University of Eastern Finland, Joensuu 80130, Finland.
University of Borås, Faculty of Textiles, Engineering and Business. (Swedish Centre for Resource Recovery)ORCID iD: 0000-0003-4887-2433
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2022 (English)In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 358, article id 127434Article in journal (Refereed) Published
Sustainable development
According to the author(s), the content of this publication falls within the area of sustainable development.
Abstract [en]

The recalcitrance of cellulosic biomass greatly hinders its enzymatic degradation. Expansins induce cell wall loosening and promote efficient cellulose utilization; however, the molecular mechanism underlying their action is not well understood. In this study, TlEXLX1, a fungal expansin from Talaromyces leycettanus JCM12802, was characterized in terms of phylogeny, synergy, structure, and mechanism of action. TlEXLX1 displayed varying degrees of synergism with commercial cellulase in the pretreatment of corn straw and filter paper. TlEXLX1 binds to cellulose via domain 2, mediated by CH–π interactions with residues Tyr291, Trp292, and Tyr327. Residues Asp237, Glu238, and Asp248 in domain 1 form hydrogen bonds with glucose units and break the inherent hydrogen bonding within the cellulose matrix. This study identified the expansin amino acid residues crucial for cellulose binding, and elucidated the structure and function of expansins in cell wall networks; this has potential applications in biomass utilization.

Place, publisher, year, edition, pages
Elsevier, 2022. Vol. 358, article id 127434
Keywords [en]
Cellulose, Expansin, Pretreatment, Cellulase, Biomass degradation, Crystallinity
National Category
Other Industrial Biotechnology
Research subject
Resource Recovery
Identifiers
URN: urn:nbn:se:hb:diva-29303DOI: 10.1016/j.biortech.2022.127434ISI: 000811461400011Scopus ID: 2-s2.0-85131688241OAI: oai:DiVA.org:hb-29303DiVA, id: diva2:1727780
Available from: 2023-01-17 Created: 2023-01-17 Last updated: 2023-01-17Bibliographically approved

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Taherzadeh, Mohammad J

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