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Enhancing or inhibitory effect of fruit or vegetable bioactive compound on Aspergillus niger and A. oryzae
University of Borås, Faculty of Textiles, Engineering and Business.
Universitas Gadjah Mada, Indonesia.
Universitas Gadjah Mada, Indonesia.
Universitas Gadjah Mada, Indonesia.
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2022 (English)In: Journal of Fungi, E-ISSN 2309-608X, Vol. 8, no 1, article id 12Article in journal (Refereed) Published
Sustainable development
According to the author(s), the content of this publication falls within the area of sustainable development.
Abstract [en]

Fruit and vegetable processing wastes are global challenges but also suitable sources with a variety of nutrients for different fermentative products using bacteria, yeast or fungi. The interaction of microorganisms with bioactive compounds in fruit waste can have inhibitory or enhancing effect on microbial growth. In this study, the antimicrobial effect of 10 bioactive compounds, including octanol, ellagic acid, (−)-epicatechin, quercetin, betanin, ascorbic acid, limonene, hexanal, car-3-ene, and myrcene in the range of 0–240 mg/L on filamentous fungi Aspergillus oryzae and Aspergillus niger were investigated. These fungi were both found to be resistant to all compounds except octanol, which can be used as a natural antifungal agent, specifically against A. oryzae and A. niger contamination. On the contrary, polyphenols (quercetin and ellagic acid), ascorbic acid, and hexanal enhanced A. niger biomass yield 28%, 7.8%, 16%, and 6%, respectively. Furthermore, 240 mg/L car-3-ene was found to increase A. oryzae biomass yield 8%, while a 9% decrease was observed at lower concentration, 24 mg/L. Similarly, up to 17% decrease of biomass yield was observed from betanin and myrcene. The resistant nature of the fungi against FPW bioactive compounds shows the potential of these fungi for further application in waste valorization. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Place, publisher, year, edition, pages
MDPI , 2022. Vol. 8, no 1, article id 12
Keywords [en]
Aspergillus, Bioactive, Filamentous fungi, Flavor, Fruit processing waste, Inhibitor
National Category
Other Industrial Biotechnology
Research subject
Resource Recovery
Identifiers
URN: urn:nbn:se:hb:diva-27168DOI: 10.3390/jof8010012ISI: 000747433700001Scopus ID: 2-s2.0-85121801445OAI: oai:DiVA.org:hb-27168DiVA, id: diva2:1626476
Funder
Swedish Agency for Economic and Regional Growth, 20201656Available from: 2022-01-11 Created: 2022-01-11 Last updated: 2024-08-30Bibliographically approved
In thesis
1. Fungi-Based Biorefinery: Valorization of industrial residuals and techno-economic evaluation
Open this publication in new window or tab >>Fungi-Based Biorefinery: Valorization of industrial residuals and techno-economic evaluation
2022 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Sustainable development of society and industry is necessary for a safer future that is being challenged by environmental pollution, climate change, and scarcity of food and nutrition. Materials considered as waste can be converted into value-added products and energy, hence regained to the economy. Filamentous fungi are saprophytic microorganisms that show great potential for bioconversion of organic waste materials into a wide range of products, including ethanol, enzymes, fungal biomass, and organic acids. In this study, industrial residuals from bioethanol plants (thin stillage), agro-industrial residuals (oat husks), and fruit-processing residuals were considered as potential substrates for filamentous fungi. Different filamentous fungi species were investigated due to their edibility and ability to produce ethanol. The aim of this study was to investigate 1) the feasibility of a biorefinery concept and contribution of fungal products to the process economy, 2) the valorization of oat husks in the fungal biorefinery and its contribution to the process economy, and 3) the effect of bioactive compounds existing in fruit residuals on filamentous fungi in a synthetic medium.

Bioconversion of thin stillage into value-added products, e.g., additional ethanol produced by filamentous fungi and edible fungal biomass for various feed/food markets, improved the process economy of a conventional bioethanol plant. Techno-economic analysis was carried out with Aspen Plus® and Aspen Process Economic Analyzer®. The economy of the conventional bioethanol plant was improved to 76% and 5.6-fold higher net present value when the fungal product was sold as fish feed and human food, respectively. When the fungal product was sold as human food, Aspergillus oryzae was more advantageous than Neurospora intermedia. It is possible to integrate lignocellulosic material into the fungi-based biorefinery and produce additional products, e.g., lignin and fungal biomass as feed/food products. Several scenarios for integrating organosolv pretreatment into the biorefinery were investigated. When protein-rich biomass was sold as feed and food, 71% and 7.9-fold higher net present value was obtained, respectively, compared to the conventional ethanol plant. Similarly, other materials, e.g., fruit residuals, can be valorized through the fungal biorefinery. However, bioactive compounds in fruit residuals have antimicrobial effects; therefore, it is essential to assess the sensitivity of fungi toward them for efficient processes. In addition to the fungi species utilized in thin stillage and oat husk valorization, two other industrially important fungi, Rhizopus oligosporus and A. niger, were tested against 10 bioactive compounds with antimicrobial properties (octanol, ellagic acid, (-)-epicatechin, quercetin, betanin, ascorbic acid, limonene, hexanal, car-3-ene, and myrcene). The bioactive compounds were added to a synthetic medium to reach final concentrations of 2.4, 24 and 240 mg/L, within their natural concentration in fruit/fruit residuals. A. oryzae, A. niger and N. intermedia were inhibited by octanol, while the latter fungus was also inhibited by D-Limonene at 240 mg/L. On the other hand, R. oligosporus was resistant to inhibitory compounds and increased the biomass yield. The highest change in biomass yield was in the presence of ellagic acid with up to a 4-fold increase. Ethanol and lactic acid yields were increased 38% and 30% in the presence of ellagic acid and betanin, respectively. Similarly, the existence of quercetin and ellagic acid, ascorbic acid, and hexanal increased the biomass yield of A. niger up to 28%.

These studies can contribute to developing efficient and feasible biorefineries, in which filamentous fungi convert the industrial residuals into value-added products.

Place, publisher, year, edition, pages
Borås: Högskolan i Borås, 2022
Series
Skrifter från Högskolan i Borås, ISSN 0280-381X ; 130
Keywords
Techno-economic analysis, Organosolv, bioethanol, filamentous fungi, food, lignin, fruit, bioactive compounds
National Category
Environmental Biotechnology
Research subject
Resource Recovery
Identifiers
urn:nbn:se:hb:diva-28588 (URN)978-91-89271-78-4 (ISBN)978-91-89271-79-1 (ISBN)
Public defence
2022-11-18, C203, Allégatan 1, Borås, 10:09 (English)
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Supervisors
Available from: 2022-10-28 Created: 2022-09-13 Last updated: 2022-10-27Bibliographically approved

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Bulkan, GülruTaherzadeh, Mohammad J

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