The influence of pig manure biochar amendment (PMBA) during the pig manure (PM) and wheat straw (WS) composting was evaluated. Five concentration of PMBA (0%, 2%, 4%, 6% and10%) were applied to explore the bacterial distributions in PM compost by 16SDNA amplicons sequencing. The results showed that the addition of 6% PMBA could significantly enhanced the bacterial community abundance compared with other composts, while control has relative less bacterial population (332 OTU). The visualization of phylogenetic tree and krona demonstrated the distinctive distribution of each composts, suggested that biochar dosages have an influence on bacterial communities’ variation during co-composting. Beta-diversity of distance matrix heat-map and principal component analysis confirmed that bacterial communities were considerably correlated with increasing PMBA. Redundancy also confirmed the similarity and discrepancy among all treatments and environmental factors. This work considered as the potential of PMBA as a booster in composting, where T4 has most plentiful bacterial community and diversity.