The present study evaluates the effectiveness of different types of biochar additives and bacterial inoculation on gaseous emission, nutrient preservation, and relevant functional bacterial community during cow manure composting. The result revealed that biochar and bacterial consortium inoculation effectively inhibited gaseous emission and improved carbon and nitrogen sequestration, remarkably enriching the abundance of the functional bacteria community. Notably, superior efficacy was found in 12% wheat straw biochar and bacterial consortium amendment composting of T6 with the lowest cumulative CO2-C and NH3-N (308.02 g and 12.71 g, respectively), minimal total C and N losses, and the highest bacterial population. Additionally, gaseous emission exhibited a strong correlation between physicochemical properties with intersection of 66.78% and a unique substrate utilizing bacterial communities. Consequently, the integrated application of biochar and bacterial consortium inoculation was suggested as an efficient method to adjust microbial activity and facilitate cellulose-rich waste degradation, enabling efficient management of organic waste from cow manure and wheat straw by composting.