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RNA Silencing of Lactate Dehydrogenase Gene in Rhizopus oryzae
University of Borås, School of Engineering.
University of Borås, School of Engineering.
2011 (English)Independent thesis Advanced level (degree of Master (One Year))Student thesis
Abstract [en]

RNA silencing with direct delivery of siRNA has been used to suppress ldhA gene expression in filamentous fungus Rhizopus oryzae. Here, for the first time we show that, introducing small interfering RNA which consequently forms silencing complexes can alter the gene expression and we report a significant reduction of lactic acid production for isolates containing short (25 nt) synthetic siRNA. In all samples lactic acid production was reduced comparing with wild types. The average concentration of lactic acid production by Rhizopus oryzae during batch fermentation process where glucose has been used as a sole carbon source, diminished from 2.06 g/l in wild types to 0.36 g/l in knockdown samples which signify 5.7 times decrease. Interestingly, the average concentration of ethanol production was increased from 0.38 g/l in wild types to 0.45 g/l in knockdown samples. In some samples we were able to report even a 10 fold decrease in lactic acid production. Since R.oryzae is capable to assimilate a wide range of carbohydrates hydrolysed from lignocellulosic material in order to produce many economically valuable bulk material such as ethanol, these results suggest that RNA silencing is a useful method for industrial biotechnology to be applied in fungus Rhizopus oryzae in order to trigger the metabolism and gene expression toward a desired product.

Place, publisher, year, edition, pages
University of Borås/School of Engineering , 2011.
Series
Magisteruppsats
Keywords [en]
rna interference, knockdown, rhizopus oryzae, lactate dehydrogenase, lactic acid, ethanol
Keywords [sv]
sirna
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:hb:diva-20404Local ID: 2320/7991OAI: oai:DiVA.org:hb-20404DiVA, id: diva2:1312338
Available from: 2019-04-30 Created: 2019-04-30

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Citation style
  • apa
  • harvard1
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