Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Extraction of chitosan from Fungal cell wall by Sulfuric acid Studying the effect of Deacetylation degree and temperature on recovery chitosan
University of Borås, School of Engineering.
2010 (English)Independent thesis Advanced level (degree of Master (One Year))Student thesis
Abstract [en]

The goal of this project is extraction of chitosan optimally by surveys of temperature changes along with 1% Sulfuric acid utilization. Microbial chitosan is isolated as a bio-component from cell wall of two kinds of Zygomycetes by some extraction methods. This project compares ability of two type Fungi (R. pulusilus and M.indicus) from Zygomycets for production of chitosan. To extract of chitosan is a combinational method with 40 %( w/w) Hydroxyl sodium for cell disruption and diluted Sulfuric acid (1% w/v) for chitosan extraction from cell wall as major chemical components. 40% NaOH is used to get different degrees of deacetylation (DD) from chitin for chitosan. In addition, it is examined 1% Sulfuric acid in a combination of temperature factor changes. It is needed dialysis for chitosan purification from bonded phosphate groups. Standard curves of acetic acid experiences for DD and water phosphate determination were accomplished. It has resulted if degree of deacetylated chitin is about 50%; it has an average lost more than 50% in 1% (v/v) Sulfuric acid, hence less recovery as a no privilege that it can be relapsed by acetone in chitosan solution. Factor of temperate in same times shows important effect on extraction yield of chitosan by 1% Sulfuric acid. Extracted chitosan in 120℃ has DD about 50%. Absolutely, its solubility will be more and it needs to an intricate solution for separation of chitosan from phosphate bonds as a major impurity by dialysis, but in 90℃, DD of chitosan is more with less solubility in water. Between two Fungi, in experienced temperatures, hence, R. pulusilus has more recovery about 0.87/AIM (g/g) in 90℃, which have more much DD than 50%, and M.indicus has 0.79/AIM (g/g) in 120℃ that it has DD about 50%.

Place, publisher, year, edition, pages
University of Borås/School of Engineering , 2010.
Series
Magisteruppsats
Keywords [en]
r.pulusilus, m.indicus, extraction, recovery, chitosan, fungal cell wall, sulfuric acid, deacetylation degree, temperature
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:hb:diva-20076Local ID: 2320/6560OAI: oai:DiVA.org:hb-20076DiVA, id: diva2:1312010
Available from: 2019-04-30 Created: 2019-04-30

Open Access in DiVA

fulltext(3489 kB)79 downloads
File information
File name FULLTEXT01.pdfFile size 3489 kBChecksum SHA-512
77df4c7b3c3ddad909539317d4f9e8e768f29fff941bd87ed2d3c5affdaa2cb7f010208a9ab14edbc8e0a95f91c79d44dd44c60af93555d778bd9866c5de5fd6
Type fulltextMimetype application/pdf

By organisation
School of Engineering
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 79 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 12 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf