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Production of recombinant keratinase for poultry feather degradation
University of Borås, School of Engineering.
University of Borås, School of Engineering.
2009 (English)Independent thesis Advanced level (degree of Master (One Year))Student thesis
Abstract [en]

The keratinase gene (kerA) of Bacillus licheniformis ATCC®53757 was PCR amplified and subsequently cloned into Bacillus megaterium expression vector; pHIS1525.SPlipA and transformed in Bacillus megaterium ATCC®14945. The kerA gene carrying the recombinant plasmid pKERHIS1525.SPlipA was expressed in Bacillus megaterium under xylose inducible promoter, purified using Ni-NTA affinity column chromatography and consequently produced an extracellular keratinase activity of 29 U ml-1 after 18 h of incubation. The recombinant strain was further examined for feather degradation of intact chicken feathers. The chopped chicken feathers were partially degraded by the recombinant strain after 3days and the total macroscopic digestion was ultimately observed after seven days resulting in a yellowish peptide rich fermentation broth.

Place, publisher, year, edition, pages
University of Borås/School of Engineering , 2009.
Series
Magisteruppsats
Keywords [en]
recombinant keratinase, bacillus megaterium, feathers degradation, expression vector
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:hb:diva-19589Local ID: 2320/5504OAI: oai:DiVA.org:hb-19589DiVA, id: diva2:1311523
Available from: 2019-04-30 Created: 2019-04-30

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Citation style
  • apa
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Output format
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