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Optimization of growth conditions for a recombinant keratinase producing bacterial strain
University of Borås, School of Engineering.
2014 (English)Independent thesis Advanced level (degree of Master (Two Years))Student thesis
Abstract [en]

A recombinant Bacillus megaterium strain with a chromosomally integrated gene kerA, expressing keratinase, was analyzed regarding action in specific environmental stresses to obtain optimum parameters necessary for bacterial growth and optimal expression of the recombinant gene. The strain was grown on chicken feathers as substrate and the degradation of feather keratin during the growth was investigated with different levels of pH, temperature and feather concentration in the broth. Two strains were analyzed, capable to grow with 575 x and 1025 x amounts of antibiotics, erythromycin, where the strain of 1025 x showed higher potential for expression. Maximum keratinolytic activity was achieved at a pH of value 8.5, at temperature of 45 °C and substrate concentration of 0.2 % (w/v). The growth rate was determined by total plate count, which indicated that there was an increasing number of colony forming units together with increasing concentration of soluble keratin. The diluted fractions did not appear to produce colonies to any significant amount. However the results for which reason the results for optimal growth conditions are guidelines for future analysis for a chromosomally recombinant Bacillus megaterium.

Place, publisher, year, edition, pages
University of Borås/School of Engineering , 2014.
Series
Masteruppsats
Keywords [en]
Keratinase, chicken feathers, growth, Bacillus megaterium
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:hb:diva-17512Local ID: 2320/13312OAI: oai:DiVA.org:hb-17512DiVA, id: diva2:1309421
Note
Program: Master of Science with a Major in Resource Recovery – Industrial BiotechnologyAvailable from: 2019-04-30 Created: 2019-04-30

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CiteExportLink to record
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Citation style
  • apa
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