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Inhibition effects of furfural on alcohol dehydrogenase, aldehyde dehydrogenase and pyruvate dehydrogenase
Dept. of Chemical Engineering II, Lund University.
Dept. of Chemical Engineering II, Lund University.
Dept. of Chemical Engineering II, Lund University.ORCID iD: 0000-0003-4887-2433
2002 (English)In: Biochemical Journal, ISSN 0264-6021, E-ISSN 1470-8728, Vol. 363, no 3, p. 769-776Article in journal (Refereed) Published
Abstract [en]

The kinetics of furfural inhibition of the enzymes alcohol dehydrogenase (ADH; EC 1.1.1.1), aldehyde dehydrogenase (A1DH; EC 1.2.1.5) and the pyruvate dehydrogenase (PDH) complex were studied in vitro. At a concentration of less than 2 mM furfural was found to decrease the activity of both PDH and A1DH by more than 90%, whereas the ADH activity decreased by less than 20% at the same concentration. Furfural inhibition of ADH and A1DH activities could be described well by a competitive inhibition model, whereas the inhibition of PDH was best described as non-competitive. The estimated Km value of A1DH for furfural was found to be about 5 μM, which was lower than that for acetaldehyde (10 μM). For ADH, however, the estimated Km value for furfural (1.2 mM) was higher than that for acetaldehyde (0.4 mM). The inhibition of the three enzymes by 5-hydroxymethylfurfural (HMF) was also measured. The inhibition caused by HMF of ADH was very similar to that caused by furfural. However, HMF did not inhibit either A1DH or PDH as severely as furfural. The inhibition effects on the three enzymes could well explain previously reported in vivo effects caused by furfural and HMF on the overall metabolism of Saccharomyces cerevisiae, suggesting a critical role of these enzymes in the observed inhibition.

Place, publisher, year, edition, pages
2002. Vol. 363, no 3, p. 769-776
Keywords [en]
A1DH, Acetaldehyde, ADH, HMF, PDH
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:hb:diva-14830DOI: 10.1042/0264-6021:3630769PubMedID: 11964178Scopus ID: 2-s2.0-0036566476ISBN: 02646021 (ISSN) OAI: oai:DiVA.org:hb-14830DiVA, id: diva2:1236475
Available from: 2018-08-02 Created: 2018-08-02 Last updated: 2018-08-08Bibliographically approved

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Taherzadeh, Mohammad J

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