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Continuous cultivation of dilute-acid hydrolysates to ethanol by immobilized Saccharomyces cerevisiae
Dept. of Chemical Reaction Eng., Chalmers University of Technology.ORCID iD: 0000-0003-4887-2433
Dept. of Chemical Reaction Eng., Chalmers University of Technology.
Dept. of Chemical Reaction Eng., Chalmers University of Technology.
2001 (English)In: Applied Biochemistry and Biotechnology, ISSN 0273-2289, E-ISSN 1559-0291, Vol. 95, no 1, p. 45-57Article in journal (Refereed) Published
Abstract [en]

The continuous cultivation of immobilized Saccharomyces cerevisiae CBS 8066 on dilute-acid hydrolysates of forest residuals was investigated. The yeast cells were immobilized in 2-4% Ca-alginate beads. The 2% beads were not stable. However, the 3 and 4% beads were stable for at least 3 wk when an extra resource of calcium ions was available in the medium. The continuous cultivation of a dilute-acid hydrolysate by the immobilized cells at dilution rates of 0.3, 0.5, and 0.6 h-1 resulted in 86, 83, and 79% sugar consumption, respectively, and an ethanol yield between 0.45 and 0.48 g/g. The hydrolysate was fermentable at a dilution rate of 0.1 h-1 in a free-cell system but washed out at a dilution rate of 0.2 h-1 The continuous cultivation of a more inhibiting hydrolysate was not successful by either free- or immobilized-cell systems even at a low dilution rate of 0.07 h-1. However, when the hydrolysate was overlimed, it was fermentable by the immobilized cells at a dilution rate of 0.2 h-1.

Place, publisher, year, edition, pages
2001. Vol. 95, no 1, p. 45-57
Keywords [en]
Dilute-acid hydrolysate, Ethanol, Glucose, Immobilization, Saccharomyces cerevisiae
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:hb:diva-14859DOI: 10.1385/ABAB:95:1:45ISI: 000171191300005PubMedID: 11665806Scopus ID: 2-s2.0-0034807097ISBN: 02732289 (ISSN) OAI: oai:DiVA.org:hb-14859DiVA, id: diva2:1236329
Available from: 2018-08-01 Created: 2018-08-01 Last updated: 2018-08-08Bibliographically approved

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Taherzadeh, Mohammad J

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