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Mycelial pellet formation by edible ascomycete filamentous fungi, Neurospora intermedia
University of Borås, Faculty of Textiles, Engineering and Business. (Swedish Centre for Resource Recovery (Biotechnology))ORCID iD: 0000-0001-5719-7252
University of Borås, Faculty of Textiles, Engineering and Business. (Swedish Centre for Resource Recovery (Biotechnology))
University of Borås, Faculty of Textiles, Engineering and Business. (Swedish Centre for Resource Recovery (Biotechnology))ORCID iD: 0000-0003-4887-2433
2016 (English)In: AMB Express, ISSN 2191-0855, E-ISSN 2191-0855, Vol. 6, no 31, 10.1186/s13568-016-0203-2Article in journal (Refereed) Published
Sustainable development
The content falls within the scope of Sustainable Development
Abstract [en]

Pellet formation of filamentous fungi in submerged culture is an imperative topic of fermentation research. In this study, we report for the first time the growth of filamentous ascomycete fungus,Neurospora intermedia in its mycelial pellet form. In submerged culture, the growth morphology of the fungus was successfully manipulated into growing as pellets by modifying various cultivation conditions. Factors such as pH (2.0–10.0), agitation rate (100–150 rpm), carbon source (glucose, arabinose, sucrose, and galactose), the presence of additive agents (glycerol and calcium chloride) and trace metals were investigated for their effect on the pellet formation. Of the various factors screened, uniform pellets were formed only at pH range 3.0–4.0, signifying it as the most influential factor for N. intermedia pellet formation. The average pellet size ranged from 2.38 ± 0.12 to 2.86 ± 0.38 mm. The pellet formation remained unaffected by the inoculum type used and its size showed an inverse correlation with the agitation rate of the culture. Efficient glucose utilization was observed with fungal pellets, as opposed to the freely suspended mycelium, proving its viability for fast- fermentation processes. Scale up of the pelletization process was also carried out in bench-scale airlift and bubble column reactors (4.5 L).

Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2016. Vol. 6, no 31, 10.1186/s13568-016-0203-2
Keyword [en]
Neurospora intermedia; Ascomycete; Filamentous fungi; Edible fungi; Pellet
National Category
Bioprocess Technology
Research subject
Resource Recovery
Identifiers
URN: urn:nbn:se:hb:diva-10462DOI: 10.1186/s13568-016-0203-2OAI: oai:DiVA.org:hb-10462DiVA: diva2:951123
Funder
Swedish Energy AgencySwedish Research Council Formas
Available from: 2016-08-05 Created: 2016-08-05 Last updated: 2017-09-22Bibliographically approved
In thesis
1. Integration of first and second generation bioethanol processes using edible filamentous fungus Neurospora intermedia
Open this publication in new window or tab >>Integration of first and second generation bioethanol processes using edible filamentous fungus Neurospora intermedia
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Establishing a commercial, lignocellulose-based, second-generation ethanol process has received several decades of attention by both researchers and industry. However, a fully economically viable process still remains a long-term goal. The main bottleneck to this achievement is the recalcitrance of lignocellulosic feedstocks, although there are several other factors, such as the huge investment required for second-generation ethanol facilities. An intelligent alternative solution discussed in this thesis is an integrated approach using firstgeneration ethanol plants for second-generation processes. Wheat is the major feedstock for first-generation ethanol in Europe; therefore, wheat-based lignocellulose waste, such as wheat straw, bran, and whole stillage fiber (a waste stream from first-generation wheat-based ethanol plants) was the primary focus of the integration model in this thesis. Since the major share of first-generation ethanol plant economics focuses on the animal feed DDGS (Distillers’ dried gains with solubles), the integration of lignocellulose should be designed in order to maintain DDGS quality. An ethanol-producing edible filamentous fungus, Neurospora intermedia, a potential protein source in DDGS, was considered for use as the fermenting microbe. The morphological and physiological aspects of this fungus were studied in the thesis, leading to the first report of fungal pellet development.

An alternative approach of using dilute phosphoric acid to pretreat lignocellulose, as it does not negatively affect fungal growth or DDGS quality, was demonstrated in both the laboratory and on a 1m3 pilot scale. Furthermore, the process of hydrolysis of pretreated lignocelluloses and subsequent N. intermedia fermentation on lignocellulose hydrolysate was also optimized in the laboratory and scaled up to 1 m3 using an in-house pilot-scale airlift bioreactor. Fungal fermentation on acid-pretreated and enzyme-hydrolyzed wheat bran, straw and whole stillage fiber resulted in a final ethanol yield of 95%, 94% and 91% of the theoretical maximum based on the glucan content of the substrate, respectively. Integrating the first- and second-generation processes using thin stillage (a waste stream from first-generation wheat-based ethanol plants) enhanced the fungal growth on straw hydrolysate, avoiding the need for supplementing with extra nutrients.

Based on the results obtained from this thesis work, a new model for integrated first- and second-generation ethanol using edible filamentous fungi processes that also adds value to animal feed (DDGS) was developed.

Place, publisher, year, edition, pages
Borås: Högskolan i Borås, 2017
Series
Skrifter från Högskolan i Borås, ISSN 0280-381X ; 82
Keyword
First- and second-generation bioethanol, Integration, Neurospora intermedia, Edible filamentous fungi, Wheat straw, Wheat bran, Whole stillage fiber
National Category
Natural Sciences
Identifiers
urn:nbn:se:hb:diva-12436 (URN)978-91-88269-51-5 (ISBN)978-91-88269-52-2 (ISBN)
Available from: 2017-09-13 Created: 2017-07-24 Last updated: 2017-09-22Bibliographically approved

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