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Dilute phosphoric acid pretreatment of wheat bran for enzymatic hydrolysis and subsequent ethanol production by edible fungi Neurospora intermedia.
University of Borås, Faculty of Textiles, Engineering and Business. Swedish Centre for Resource Recovery. (Biotechnology)ORCID iD: 0000-0001-5719-7252
University of Borås, Faculty of Textiles, Engineering and Business. Swedish Centre for Resource Recovery. (Biotechnology)
University of Borås, Faculty of Textiles, Engineering and Business. Swedish Centre for Resource Recovery. (Biotechnology)
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2015 (English)In: Industrial crops and products (Print), ISSN 0926-6690, E-ISSN 1872-633X, Vol. 69, 314-323 p.Article in journal (Refereed) Published
Abstract [en]

The use of an underutilized and abundant lignocellulosic feedstock residue, wheat bran, was studied for ethanol prodn. using dil. phosphoric acid pretreatment followed by fermn. using edible fungi Neurospora intermedia. Wheat bran was subjected to dil. acid pretreatment at varying acid concns. (0.5-3.0% w/v), temp. (150-210 °C), and reaction time (5-20 min). The interaction of multiple factors showed the optimum pretreatment conditions at acid concn. of 1.75% (w/v), at 190 °C for 10 min. The max. total polysaccharide yield of 0.27 ± 0.01 g/g dry biomass loading, corresponding to 66% of the theor. max. was obsd. Subsequent fermn. with N. intermedia showed 85% of the theor. max. ethanol yield from the untreated bran glucose. The effect of the dil. acid pretreatment on the functional groups of the wheat bran cellulose was detd. with 78% redn. in the cellulose crystallinity index. The validation of the dil. phosphoric acid pretreatment in a demo plant is also reported for the first time. Enzymic hydrolysis of pretreated slurry from the demo plant showed 85% total theor. yield of polysaccharides. Compared to the untreated bran biomass, an increase of 51% was obsd. in the ethanol yield following pretreatment, with a total ethanol yield of 95% theor. max. Higher yield of ethanol is also attributed to the xylose fermenting capability of the fungi. [on SciFinder(R)]

Place, publisher, year, edition, pages
2015. Vol. 69, 314-323 p.
Keyword [en]
Dilute phosphoric acid, Edible fungi, Ethanol fermentation, Neurospora intermedia, Pretreatment, Wheat bran
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:hb:diva-88DOI: 10.1016/j.indcrop.2015.02.038ISI: 000355365300041Scopus ID: 84923361612OAI: oai:DiVA.org:hb-88DiVA: diva2:877299
Available from: 2015-12-06 Created: 2015-05-22 Last updated: 2017-09-22Bibliographically approved
In thesis
1. Integration of first and second generation bioethanol processes using edible filamentous fungus Neurospora intermedia
Open this publication in new window or tab >>Integration of first and second generation bioethanol processes using edible filamentous fungus Neurospora intermedia
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Establishing a commercial, lignocellulose-based, second-generation ethanol process has received several decades of attention by both researchers and industry. However, a fully economically viable process still remains a long-term goal. The main bottleneck to this achievement is the recalcitrance of lignocellulosic feedstocks, although there are several other factors, such as the huge investment required for second-generation ethanol facilities. An intelligent alternative solution discussed in this thesis is an integrated approach using firstgeneration ethanol plants for second-generation processes. Wheat is the major feedstock for first-generation ethanol in Europe; therefore, wheat-based lignocellulose waste, such as wheat straw, bran, and whole stillage fiber (a waste stream from first-generation wheat-based ethanol plants) was the primary focus of the integration model in this thesis. Since the major share of first-generation ethanol plant economics focuses on the animal feed DDGS (Distillers’ dried gains with solubles), the integration of lignocellulose should be designed in order to maintain DDGS quality. An ethanol-producing edible filamentous fungus, Neurospora intermedia, a potential protein source in DDGS, was considered for use as the fermenting microbe. The morphological and physiological aspects of this fungus were studied in the thesis, leading to the first report of fungal pellet development.

An alternative approach of using dilute phosphoric acid to pretreat lignocellulose, as it does not negatively affect fungal growth or DDGS quality, was demonstrated in both the laboratory and on a 1m3 pilot scale. Furthermore, the process of hydrolysis of pretreated lignocelluloses and subsequent N. intermedia fermentation on lignocellulose hydrolysate was also optimized in the laboratory and scaled up to 1 m3 using an in-house pilot-scale airlift bioreactor. Fungal fermentation on acid-pretreated and enzyme-hydrolyzed wheat bran, straw and whole stillage fiber resulted in a final ethanol yield of 95%, 94% and 91% of the theoretical maximum based on the glucan content of the substrate, respectively. Integrating the first- and second-generation processes using thin stillage (a waste stream from first-generation wheat-based ethanol plants) enhanced the fungal growth on straw hydrolysate, avoiding the need for supplementing with extra nutrients.

Based on the results obtained from this thesis work, a new model for integrated first- and second-generation ethanol using edible filamentous fungi processes that also adds value to animal feed (DDGS) was developed.

Place, publisher, year, edition, pages
Borås: Högskolan i Borås, 2017
Series
Skrifter från Högskolan i Borås, ISSN 0280-381X ; 82
Keyword
First- and second-generation bioethanol, Integration, Neurospora intermedia, Edible filamentous fungi, Wheat straw, Wheat bran, Whole stillage fiber
National Category
Natural Sciences
Identifiers
urn:nbn:se:hb:diva-12436 (URN)978-91-88269-51-5 (ISBN)978-91-88269-52-2 (ISBN)
Available from: 2017-09-13 Created: 2017-07-24 Last updated: 2017-09-22Bibliographically approved

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Nair, RamkumarLundin, MagnusLennartsson, PatrikTaherzadeh, Mohammad J
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