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Determination of glucosamine and N-acetyl glucosamine in fungal cell walls
Högskolan i Borås, Institutionen Ingenjörshögskolan.
Högskolan i Borås, Institutionen Ingenjörshögskolan.
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2008 (Engelska)Ingår i: Journal of Agricultural and Food Chemistry, ISSN 0021-8561, E-ISSN 1520-5118, Vol. 56, nr 18, s. 8314-8318Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A new method was developed to determine glucosamine (GlcN) and N-acetyl glucosamine (GlcNAc) in materials containing chitin and chitosan, such as fungal cell walls. It is based on two steps of hydrolysis with (i) concentrated sulfuric acid at low temperature and (ii) dilute sulfuric acid at high temperature, followed by one-step degradation with nitrous acid. In this process, chitin and chitosan are converted into anhydromannose and acetic acid. Anhydromannose represents the sum of GlcN and GlcNAc, whereas acetic acid is a marker for GlcNAc only. The method showed recovery of 90.1% of chitin and 85.7-92.4% of chitosan from commercial preparations. Furthermore, alkali insoluble material (AIM) from biomass of three strains of zygomycetes, Rhizopus oryzae, Mucor indicus, and Rhizomucor pusillus, was analyzed by this method. The glucosamine contents of AIM from R. oryzae and M. indicus were almost constant (41.7 +/- 2.2% and 42.0 +/- 1.7%, respectively), while in R. pusillus, it decreased from 40.0 to 30.0% during cultivation from 1 to 6 days. The GlcNAc content of AIM from R. oryzae and R. pusillus increased from 24.9 to 31.0% and from 36.3 to 50.8%, respectively, in 6 days, while it remained almost constant during the cultivation of M. indicus (23.5 +/- 0.8%).

Ort, förlag, år, upplaga, sidor
American Chemical Society , 2008. Vol. 56, nr 18, s. 8314-8318
Nyckelord [en]
alkali-insoluble material, chitin, chitosan, fungal cell wall, glucosamine, n-acetyl glucosamine, nitrous acid, sulfuric acid, AIM, glcn, glcnac
Nyckelord [sv]
Energi och material
Nationell ämneskategori
Annan medicinsk grundvetenskap Biologiska vetenskaper
Identifikatorer
URN: urn:nbn:se:hb:diva-2550DOI: 10.1021/jf801478jLokalt ID: 2320/4505OAI: oai:DiVA.org:hb-2550DiVA, id: diva2:870644
Tillgänglig från: 2015-11-13 Skapad: 2015-11-13 Senast uppdaterad: 2018-01-10Bibliografiskt granskad

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Zamani, AkramJeihanipour, AzamTaherzadeh, Mohammad J.

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Zamani, AkramJeihanipour, AzamTaherzadeh, Mohammad J.
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Institutionen Ingenjörshögskolan
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Journal of Agricultural and Food Chemistry
Annan medicinsk grundvetenskapBiologiska vetenskaper

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