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Piezoelectric inkjet printing of tyrosinase (polyphenol oxidase) enzyme on atmospheric plasma treated polyamide fabric
Högskolan i Borås, Akademin för textil, teknik och ekonomi.ORCID-id: 0000-0003-2412-9004
Högskolan i Borås, Akademin för textil, teknik och ekonomi.ORCID-id: 0000-0002-1008-1313
Högskolan i Borås, Akademin för textil, teknik och ekonomi.ORCID-id: 0000-0002-4369-9304
2022 (engelsk)Inngår i: Scientific Reports, E-ISSN 2045-2322, Vol. 12, nr 1, artikkel-id 6828Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Tyrosinase enzyme was digitally printed on plasma pretreated polyamide-6,6 fabric using several sustainable technologies. Ink containing carboxymethyl cellulose was found to be the most suitable viscosity modifier for this enzyme. Before and after being deposited on the fabric surface, the printed inks retained enzyme activity of 69% and 60%, respectively, compared to activity prior printing process. A good number of the printed enzyme was found to be strongly adsorbed on the fabric surface even after several rinsing cycles due to surface activation by plasma treatment. Rinsed out fabrics retained a maximum activity of 34% resulting from the well-adsorbed enzymes. The activity of tyrosinase on printed fabrics was more stable than ink solution for at least 60 days. Effects of pH, temperature and enzyme kinetics on ink solution and printed fabrics were assessed. Tyrosinase printed synthetic fabrics can be utilized for a range of applications from biosensing and wastewater treatment to cultural heritage works.

sted, utgiver, år, opplag, sider
2022. Vol. 12, nr 1, artikkel-id 6828
Emneord [en]
covalent immobilization, biosensor, adsorption
HSV kategori
Forskningsprogram
Textil och mode (generell)
Identifikatorer
URN: urn:nbn:se:hb:diva-27875DOI: 10.1038/s41598-022-10852-2ISI: 000787775900070Scopus ID: 2-s2.0-85128933473OAI: oai:DiVA.org:hb-27875DiVA, id: diva2:1658535
Tilgjengelig fra: 2022-05-16 Laget: 2022-05-16 Sist oppdatert: 2023-01-11bibliografisk kontrollert
Inngår i avhandling
1. Enzyme Printed Fabrics: Bio‐functionalisation of Synthetic Textiles by Digital Inkjet Printing
Åpne denne publikasjonen i ny fane eller vindu >>Enzyme Printed Fabrics: Bio‐functionalisation of Synthetic Textiles by Digital Inkjet Printing
2022 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

This thesis explores the possibilities of printing enzymes using resource-efficient technologies to promote the binding of other proteins and biomaterials on synthetic textiles. This strategy can be used to develop advanced textiles for applications, for example, in antimicrobial, drug delivery and biosensing. Digital inkjet printing was combined with enzyme technology to ensure minimum use of water, chemicals and energy in textile manufacturing processes.  

Inks containing two enzymes, lysozyme and tyrosinase, were formulated by adjusting several rheological and ionic properties. The activity of these enzymes was optimised while being printed through two different industrial grade piezoelectric printheads. The theoretical printability of the prepared inks was calculated. The effect of printhead temperature and number of printing passes on the activity was evaluated. Polyester (polyethylene terephthalate) and polyamide-6,6 were pre-treated through several techniques to understand their effect on enzyme adhesion, binding and activity retention. Tyrosinase was used to bind lysozyme on plasma activated polyamide-6,6 surface. The effects of printing these two enzymes in various sequences, i.e. tyrosinase before lysozyme and vice-versa on binding stability and activity, were studied. Influence of the printing process on enzyme kinetics was evaluated. Ability to store and reuse printed fabrics was also studied.  

Lysozyme and tyrosinase containing inks showed activity retention of 85% and 60%, respectively. Activity of lysozyme containing ink was optimum at 10–15 mPa.s when glycerol was used as a viscosity modifier. However, the optimum viscosity for tyrosinase containing ink was at 6–9 mPa.s, and carboxymethyl cellulose was found to be the most favourable modifier. For both inks, a surfactant amount below the critical micelle concentration was considered to be the most effective for printing. Among the studied fabric pre-treatment methods (alkaline, cutinase and plasma), it was found that the activity and stability of the enzyme were dependent on the nature of the pretreatment processes, which can be beneficial for different application areas, e.g. drug release and bio-sensing. Upon printing both inks on a plasma treated polyamide-6,6, tyrosinase was able to catalyse lysozyme protein to bind it on fabric. A maximum of 68% lytic activity was retained by lysozyme when it was printed after tyrosinase. This fabric showed inhibition of bacterial growth and retained almost half of its initial activity when cold stored for a month. 

sted, utgiver, år, opplag, sider
Borås: Högskolan i Borås, 2022
Serie
Skrifter från Högskolan i Borås, ISSN 0280-381X ; 135
Emneord
Digital printing, inkjet, enzyme, printhead, rheology, immobilisation, piezoelectric, drop-on-demand, resource-efficient textiles, biological ink, lysozyme, tyrosinase, polyphenol oxidase, polyethylene terephthalate, polyester, polyamide-6, 6, nylon, surface modification, plasma, antimicrobial, antibacterial
HSV kategori
Forskningsprogram
Textil och mode (generell)
Identifikatorer
urn:nbn:se:hb:diva-29001 (URN)978-91-89271-87-6 (ISBN)978-91-89271-88-3 (ISBN)
Disputas
2023-02-28, M404, Allégatan 1, Borås, 09:00 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2023-01-31 Laget: 2022-12-01 Sist oppdatert: 2023-03-06bibliografisk kontrollert

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Biswas, TuserYu, JunchunNierstrasz, Vincent

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