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Piezoelectric inkjet printing of tyrosinase (polyphenol oxidase) enzyme on atmospheric plasma treated polyamide fabric
University of Borås, Faculty of Textiles, Engineering and Business.ORCID iD: 0000-0003-2412-9004
University of Borås, Faculty of Textiles, Engineering and Business.ORCID iD: 0000-0002-1008-1313
University of Borås, Faculty of Textiles, Engineering and Business.ORCID iD: 0000-0002-4369-9304
2022 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 12, no 1, article id 6828Article in journal (Refereed) Published
Abstract [en]

Tyrosinase enzyme was digitally printed on plasma pretreated polyamide-6,6 fabric using several sustainable technologies. Ink containing carboxymethyl cellulose was found to be the most suitable viscosity modifier for this enzyme. Before and after being deposited on the fabric surface, the printed inks retained enzyme activity of 69% and 60%, respectively, compared to activity prior printing process. A good number of the printed enzyme was found to be strongly adsorbed on the fabric surface even after several rinsing cycles due to surface activation by plasma treatment. Rinsed out fabrics retained a maximum activity of 34% resulting from the well-adsorbed enzymes. The activity of tyrosinase on printed fabrics was more stable than ink solution for at least 60 days. Effects of pH, temperature and enzyme kinetics on ink solution and printed fabrics were assessed. Tyrosinase printed synthetic fabrics can be utilized for a range of applications from biosensing and wastewater treatment to cultural heritage works.

Place, publisher, year, edition, pages
2022. Vol. 12, no 1, article id 6828
Keywords [en]
covalent immobilization, biosensor, adsorption
National Category
Textile, Rubber and Polymeric Materials
Research subject
Textiles and Fashion (General)
Identifiers
URN: urn:nbn:se:hb:diva-27875DOI: 10.1038/s41598-022-10852-2ISI: 000787775900070Scopus ID: 2-s2.0-85128933473OAI: oai:DiVA.org:hb-27875DiVA, id: diva2:1658535
Available from: 2022-05-16 Created: 2022-05-16 Last updated: 2023-01-11Bibliographically approved
In thesis
1. Enzyme Printed Fabrics: Bio‐functionalisation of Synthetic Textiles by Digital Inkjet Printing
Open this publication in new window or tab >>Enzyme Printed Fabrics: Bio‐functionalisation of Synthetic Textiles by Digital Inkjet Printing
2022 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis explores the possibilities of printing enzymes using resource-efficient technologies to promote the binding of other proteins and biomaterials on synthetic textiles. This strategy can be used to develop advanced textiles for applications, for example, in antimicrobial, drug delivery and biosensing. Digital inkjet printing was combined with enzyme technology to ensure minimum use of water, chemicals and energy in textile manufacturing processes.  

Inks containing two enzymes, lysozyme and tyrosinase, were formulated by adjusting several rheological and ionic properties. The activity of these enzymes was optimised while being printed through two different industrial grade piezoelectric printheads. The theoretical printability of the prepared inks was calculated. The effect of printhead temperature and number of printing passes on the activity was evaluated. Polyester (polyethylene terephthalate) and polyamide-6,6 were pre-treated through several techniques to understand their effect on enzyme adhesion, binding and activity retention. Tyrosinase was used to bind lysozyme on plasma activated polyamide-6,6 surface. The effects of printing these two enzymes in various sequences, i.e. tyrosinase before lysozyme and vice-versa on binding stability and activity, were studied. Influence of the printing process on enzyme kinetics was evaluated. Ability to store and reuse printed fabrics was also studied.  

Lysozyme and tyrosinase containing inks showed activity retention of 85% and 60%, respectively. Activity of lysozyme containing ink was optimum at 10–15 mPa.s when glycerol was used as a viscosity modifier. However, the optimum viscosity for tyrosinase containing ink was at 6–9 mPa.s, and carboxymethyl cellulose was found to be the most favourable modifier. For both inks, a surfactant amount below the critical micelle concentration was considered to be the most effective for printing. Among the studied fabric pre-treatment methods (alkaline, cutinase and plasma), it was found that the activity and stability of the enzyme were dependent on the nature of the pretreatment processes, which can be beneficial for different application areas, e.g. drug release and bio-sensing. Upon printing both inks on a plasma treated polyamide-6,6, tyrosinase was able to catalyse lysozyme protein to bind it on fabric. A maximum of 68% lytic activity was retained by lysozyme when it was printed after tyrosinase. This fabric showed inhibition of bacterial growth and retained almost half of its initial activity when cold stored for a month. 

Place, publisher, year, edition, pages
Borås: Högskolan i Borås, 2022
Series
Skrifter från Högskolan i Borås, ISSN 0280-381X ; 135
Keywords
Digital printing, inkjet, enzyme, printhead, rheology, immobilisation, piezoelectric, drop-on-demand, resource-efficient textiles, biological ink, lysozyme, tyrosinase, polyphenol oxidase, polyethylene terephthalate, polyester, polyamide-6, 6, nylon, surface modification, plasma, antimicrobial, antibacterial
National Category
Textile, Rubber and Polymeric Materials
Research subject
Textiles and Fashion (General)
Identifiers
urn:nbn:se:hb:diva-29001 (URN)978-91-89271-87-6 (ISBN)978-91-89271-88-3 (ISBN)
Public defence
2023-02-28, M404, Allégatan 1, Borås, 09:00 (English)
Opponent
Supervisors
Available from: 2023-01-31 Created: 2022-12-01 Last updated: 2023-03-06Bibliographically approved

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Biswas, TuserYu, JunchunNierstrasz, Vincent

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