It has been suggested that cellulolytic enzymes can be effective on the degradation of PLA samples. The idea was investigated by examining the impact of cellulase on degradation of PLA and PLA-jute (64/36) composite in an aqueous medium. The obtained results demonstrated 55% and 61% thickness reduction in PLA and PLA-jute specimens after four months of treatment, respectively. Gel permeation chromatography (GPC) showed significant decline in the number average molecular weight (Mn) approximately equal to 85% and 80% for PLA and PLA-jute in comparison with their control. The poly dispersity index (PDI) of PLA and PLA-jute declined 41% and 49% that disclosed more homogenous distribution in molecular weight of the polymer after treatment with cellulase. The cellulase promiscuity effect on PLA degradation was further revealed by Fourier-transform infrared spectroscopy (FT-IR) analysis where substantial decrease in the peak intensities of the polymer related functional groups were observed. In addition, PLA biodegradation was studied in more detail by differential scanning calorimetry (DSC) and thermal gravimetric analysis (TGA) of control and cellulase treated specimens. The obtained results confirmed the promiscuous function of cellulase in the presence or the absence of jute as the specific substrate of cellulase. This can be considered as a major breakthrough to develop effective biodegradation processes for PLA products at the end of their life cycle.