Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • harvard-cite-them-right
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Toward Bioluminescent Materials by Plasma Treatment of Microfibrous Nonwovens, Followed by Immobilization of One or Both Enzyme(s) (Luciferase and FMN Reductase) Involved in Luminescent Bacteria
Högskolan i Borås, Akademin för textil, teknik och ekonomi. (Textile technology)ORCID-id: 0000-0001-9671-1242
ENSAIT.
Soochow university.
Högskolan i Borås, Akademin för textil, teknik och ekonomi.ORCID-id: 0000-0002-4369-9304
2020 (Engelska)Ingår i: ACS Applied Bio Materials, E-ISSN 2576-6422Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Bioluminescent living organisms emit light through a specific biocatalyzed reaction involving a luciferin substrate and a luciferase enzyme. The present work investigated the possibility of creating optimal luminescence by immobilization of one or both the enzymes Luciferase (Luc) and FMN reductase (Red) involved in a bioluminescent bacterial system onto a plasma-activated microfibrous PET nonwoven. Parameters affecting the catalytic activity and efficiency of the bacterial system in aqueous medium were determined by luminescence intensity measurements using a luminometer. Two types of plasma, air atmospheric plasma (ATMP) and cold remote plasma (CRPNO) treatment, were used to activate the PET nonwoven. Further, one or both enzyme(s) were immobilized using a physical adsorption technique, without or with the use of natural biopolymers (gelatin and starch) and bovine serum albumin-BSA protein, to improve enzyme stability and activity. Coimmobilization of both Red and Luc enzymes on the CRPNO plasma-activated nonwoven in the presence of BSA led to the maximum luminescence. As high as 60,000 RLU equivalent to that of an LED light used for calibration was observed and showed stable intensity up to 6 min. Fiber surface analysis was tested using wettability tests (water contact angle and capillary uptake), while scanning electron microscopy, atomic force microscopy, and electron spectroscopy for chemical analysis showed changes in fiber surface morphology and chemical functional groups. A considerable increase in “N” atom content after coimmobilization of enzymes in the presence of BSA was detected. This study is the first successful attempt to use a biomimetic strategy for immobilization of enzymes involved in bacterial luminescence on a plasma-activated microfibrous nonwoven in an attempt to attain bioluminescent materials.

Ort, förlag, år, upplaga, sidor
2020.
Nationell ämneskategori
Teknik och teknologier
Identifikatorer
URN: urn:nbn:se:hb:diva-23165DOI: 10.1021/acsabm.0c00329ISI: 000604588000086Scopus ID: 2-s2.0-85085841118OAI: oai:DiVA.org:hb-23165DiVA, id: diva2:1428168
Tillgänglig från: 2020-05-04 Skapad: 2020-05-04 Senast uppdaterad: 2022-01-20Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextScopus

Person

Iyer, SwetaNierstrasz, Vincent

Sök vidare i DiVA

Av författaren/redaktören
Iyer, SwetaNierstrasz, Vincent
Av organisationen
Akademin för textil, teknik och ekonomi
I samma tidskrift
ACS Applied Bio Materials
Teknik och teknologier

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 1548 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • harvard-cite-them-right
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf