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Optimizing dilute phosphoric acid pretreatment of wheat straw in the laboratory and in a demonstration plant for ethanol and edible fungal biomass production using Neurospora intermedia.
University of Borås, Faculty of Textiles, Engineering and Business. (Resource Recovery)
University of Borås, Faculty of Textiles, Engineering and Business. (Resource Recovery)
University of Borås, Faculty of Textiles, Engineering and Business. (Resource Recovery)
University of Borås, Faculty of Textiles, Engineering and Business. (Resource Recovery)ORCID iD: 0000-0003-4887-2433
2016 (English)In: Journal of chemical technology and biotechnology (1986), ISSN 0268-2575, E-ISSN 1097-4660Article in journal (Refereed) Published
Abstract [en]

BACKGROUND : A method is described that uses dil. phosphoric acid for wheat straw pretreatment and subsequent ethanol and fungal biomass prodn. with the edible fungus Neurospora intermedia. Dil. phosphoric acid pretreatment of wheat straw was optimized at a lab. scale, and the results were validated in a biorefinery demonstration plant for the first time. The various conditions for the dil. acid pretreatment include such factors as phosphoric acid concns. (0.5-3.0% w/v), temp. (150-210 °C), and reaction time (5-20 min). RESULTS : The optimal pretreatment conditions were detd. as an acid concn. of 1.75% (w/v) at a temp. of 190 °C for 15 min, based on the max. enzymic digestibility with the min. inhibitor release. The efficiency of enzymic polysaccharide hydrolysis was 36% for untreated straw and 86% for straw pretreated with dil. phosphoric acid. Scale up of the pretreatment at a biorefinery demonstration plant improved the process, with the subsequent efficiency of polysaccharide hydrolysis being 95% of the theor. max. Ethanol fermn. of enzymically hydrolyzed wheat straw using N. intermedia showed an improvement in the ethanol yield from 29% (with untreated straw) to 94% (with dil. phosphoric acid pretreated straw) of the theor. max. CONCLUSION : This study opens up an alternative strategy for the efficient use of wheat straw for the prodn. of ethanol and edible fungal biomass in existing wheat-to-ethanol plants.

Place, publisher, year, edition, pages
2016.
Keyword [en]
Dilute phosphoric acid, Ethanol, Fermentation, Filamentous fungi, Optimization, Pretreatment
National Category
Industrial Biotechnology
Identifiers
URN: urn:nbn:se:hb:diva-11393DOI: 10.1002/jctb.5119Scopus ID: 84997724781OAI: oai:DiVA.org:hb-11393DiVA: diva2:1056196
Note

CAPLUS AN 2016:1751717(Journal)

Available from: 2016-12-14 Created: 2016-12-14 Last updated: 2017-09-22Bibliographically approved
In thesis
1. Integration of first and second generation bioethanol processes using edible filamentous fungus Neurospora intermedia
Open this publication in new window or tab >>Integration of first and second generation bioethanol processes using edible filamentous fungus Neurospora intermedia
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Establishing a commercial, lignocellulose-based, second-generation ethanol process has received several decades of attention by both researchers and industry. However, a fully economically viable process still remains a long-term goal. The main bottleneck to this achievement is the recalcitrance of lignocellulosic feedstocks, although there are several other factors, such as the huge investment required for second-generation ethanol facilities. An intelligent alternative solution discussed in this thesis is an integrated approach using firstgeneration ethanol plants for second-generation processes. Wheat is the major feedstock for first-generation ethanol in Europe; therefore, wheat-based lignocellulose waste, such as wheat straw, bran, and whole stillage fiber (a waste stream from first-generation wheat-based ethanol plants) was the primary focus of the integration model in this thesis. Since the major share of first-generation ethanol plant economics focuses on the animal feed DDGS (Distillers’ dried gains with solubles), the integration of lignocellulose should be designed in order to maintain DDGS quality. An ethanol-producing edible filamentous fungus, Neurospora intermedia, a potential protein source in DDGS, was considered for use as the fermenting microbe. The morphological and physiological aspects of this fungus were studied in the thesis, leading to the first report of fungal pellet development.

An alternative approach of using dilute phosphoric acid to pretreat lignocellulose, as it does not negatively affect fungal growth or DDGS quality, was demonstrated in both the laboratory and on a 1m3 pilot scale. Furthermore, the process of hydrolysis of pretreated lignocelluloses and subsequent N. intermedia fermentation on lignocellulose hydrolysate was also optimized in the laboratory and scaled up to 1 m3 using an in-house pilot-scale airlift bioreactor. Fungal fermentation on acid-pretreated and enzyme-hydrolyzed wheat bran, straw and whole stillage fiber resulted in a final ethanol yield of 95%, 94% and 91% of the theoretical maximum based on the glucan content of the substrate, respectively. Integrating the first- and second-generation processes using thin stillage (a waste stream from first-generation wheat-based ethanol plants) enhanced the fungal growth on straw hydrolysate, avoiding the need for supplementing with extra nutrients.

Based on the results obtained from this thesis work, a new model for integrated first- and second-generation ethanol using edible filamentous fungi processes that also adds value to animal feed (DDGS) was developed.

Place, publisher, year, edition, pages
Borås: Högskolan i Borås, 2017
Series
Skrifter från Högskolan i Borås, ISSN 0280-381X ; 82
Keyword
First- and second-generation bioethanol, Integration, Neurospora intermedia, Edible filamentous fungi, Wheat straw, Wheat bran, Whole stillage fiber
National Category
Natural Sciences
Identifiers
urn:nbn:se:hb:diva-12436 (URN)978-91-88269-51-5 (ISBN)978-91-88269-52-2 (ISBN)
Available from: 2017-09-13 Created: 2017-07-24 Last updated: 2017-09-22Bibliographically approved

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Nair, Ramkumar B.Lundin, MagnusLennartsson, Patrik R.Taherzadeh, Mohammad J
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