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Surface modification of carbon felt by cold remote plasma for glucose oxidase enzyme immobilization
Högskolan i Borås, Akademin för textil, teknik och ekonomi. ENSAIT. (TMT)ORCID-id: 0000-0002-0507-9606
Université de Lille, Nord de France, France.
Université de Lille, Nord de France, France.
Université de Lille, Nord de France, France.
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2019 (Engelska)Ingår i: Applied Surface Science, ISSN 0169-4332, E-ISSN 1873-5584, Vol. 467, s. 1016-1024Artikel i tidskrift (Refereegranskat) In press
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Abstract [en]

Despite their chemical inertness and poor hydrophobicity, carbon-based materials are widely used in electrochemical applications due to their robustness, good electrical conductivity and corrosion resistance. The purpose of the work carried was to increase the wettability of nonwoven carbon fiber felts for improved efficiency in bio/electrochemical applications. Virgin Carbon Felt (VCF) was first treated with cold remote plasma (CRP) using a mixture of nitrogen and oxygen (1 or 2 %) as plasma gas.  Bio-functionalization of the carbon felts with glucose oxidase (GOx) enzyme was then carried using physical adsorption method. FTIR and XPS analysis showed an integration of new oxygenated functional groups (C-O and C=O) as well as amines and amides on the surface of VCF treated by the CRP treatment, which improved the wettability of the samples. Capillary uptake increased from around 0 % (for VCF) to nearly 750 % with 2 % oxygen in plasma gas. GOx enzyme showed higher activity after immobilization at pH 5.5 on the CRP treated samples, maintaining up to 50 % of its initial enzymatic activity after six cycles while with the VCF, no enzymatic activity was observed after the fourth cycle. These obtained felts can be used as electrodes in sustainable bioprocesses.

Ort, förlag, år, upplaga, sidor
Elsevier, 2019. Vol. 467, s. 1016-1024
Nyckelord [en]
Carbon felt, Cold remote plasma, Surface modification, Glucose oxidase immobilization, Enzyme activity
Nationell ämneskategori
Teknik och teknologier
Forskningsämne
Textil och mode (generell)
Identifikatorer
URN: urn:nbn:se:hb:diva-15760DOI: 10.1016/j.apsusc.2019.01.155ISI: 000459458600120Scopus ID: 2-s2.0-85060727203OAI: oai:DiVA.org:hb-15760DiVA, id: diva2:1289310
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SMDTexTillgänglig från: 2019-02-17 Skapad: 2019-02-17 Senast uppdaterad: 2020-01-29Bibliografiskt granskad

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Kahoush, MayNierstrasz, Vincent

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