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Taherzadeh, Mohammad JORCID iD iconorcid.org/0000-0003-4887-2433
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Publications (10 of 604) Show all publications
Duan, Y., Wang, Z., Ganeshan, P., Sar, T., Xu, S., Rajendran, K., . . . Awasthi, M. K. (2025). Anaerobic digestion in global bio-energy production for sustainable bioeconomy: Potential and research challenges. Renewable & sustainable energy reviews, 208, Article ID 114985.
Open this publication in new window or tab >>Anaerobic digestion in global bio-energy production for sustainable bioeconomy: Potential and research challenges
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2025 (English)In: Renewable & sustainable energy reviews, ISSN 1364-0321, E-ISSN 1879-0690, Vol. 208, article id 114985Article in journal (Refereed) Published
Abstract [en]

Wastes are unceasingly generated in the world, and most of them can be recycled, reused, or recovered to promote a circular economy. Among waste treatment approaches, the anaerobic digestion (AD) process has been considered as an ideal process due to its ecological benefits (reduction of unpleasant odor, pathogens accumulation, or greenhouse gas emission), social and economic advantages, and energy saving. In addition to biogas production, this process can be used to produce various bioproducts, such as biopolymers, bioplastics, biomass, biofertilizers, and biolipids. Interestingly, the AD process residue or digestate is a nutrient-rich by-product that can be used as a biofertilizer for agronomical purposes to balance N-P cycle in the soils. Despite of numerous benefits of AD, less than 1 % of waste is treated by this process. This process has the potential to be integrated with other waste treatment approaches to increase waste treatment efficiency. Therefore, it is essential to focus on each advantage and clarify ambiguity in order to satisfy more countries for employing AD for waste treatment. In this review, various benefits of AD are evaluated; and its potential impacts on particularly agriculture are examined in detail. Additionally, potential biomass and wastes that can be used for anaerobic digestion worldwide are deliberated. Besides, a critical perspective has been developed on the economic, environmental, and social evaluation of the benefits of AD and, as a final point, focused on an integrated circular cascading approach. 

Keywords
Anaerobic digestion, Bioeconomy, Ecological benefits, Sustainability, CO2 reduction, Bioproduct
National Category
Environmental Sciences
Research subject
Resource Recovery
Identifiers
urn:nbn:se:hb:diva-32838 (URN)10.1016/j.rser.2024.114985 (DOI)001339116800001 ()
Available from: 2024-11-22 Created: 2024-11-22 Last updated: 2024-11-28Bibliographically approved
Marques, A. M., Oliveira, S. M., Martins, A. J., Bourbon, A. I., Rousta, N., Taherzadeh, M. J., . . . Pastrana, L. M. (2025). Use of a coaxial nozzle-assisted 3D printing and ionic crosslinking to control the texture of a mycoprotein-based food product. Future Foods, 11, Article ID 100530.
Open this publication in new window or tab >>Use of a coaxial nozzle-assisted 3D printing and ionic crosslinking to control the texture of a mycoprotein-based food product
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2025 (English)In: Future Foods, ISSN 2666-8335, Vol. 11, article id 100530Article in journal (Refereed) Published
Abstract [en]

This work innovatively merges engineered food structures with additive manufacturing technology to tailor food for different dysphagia levels using filamentous Fungus Aspergillus oryzae biomass through a co-axial 3D food printing process. In order to 3D print samples with different textures, calcium chloride (CaCl2) and calcium gluconolactate (Gluco) were tested at different concentrations alongside three alginate concentrations. The structural and morphological analysis of the printed samples was conducted before cooking, followed by texture analysis and the measurement of cutting strength on cooked samples. Dysphagia food level classification was measured following the International Dysphagia Diet Standardisation Initiative (IDDSI) guidelines. Results revealed that Gluco minimally altered texture but affected appearance, resulting in soft samples (hardness ≈ 1.3 N) with fibrous structures, while CaCl2 increased hardness (from ≈ 1 N to 4.68 N) through alginate-ion Ca2+ interaction. All samples passed the IDDSI test, confirming suitability for dysphagic individuals. This successfully engineered personalised food with tailored texture meeting nutritional requirements. 

Keywords
3D food printing, alternative proteins, dysphagia food, mycoprotein, texture personalisation
National Category
Food Science
Research subject
Resource Recovery
Identifiers
urn:nbn:se:hb:diva-33033 (URN)10.1016/j.fufo.2024.100530 (DOI)2-s2.0-85213288676 (Scopus ID)
Note

The author Arlete M. Marques (SFRH/BD/132911/2017) is the recipient of a fellowship from Funda\u00E7\u00E3o para a Ci\u00EAncia e Tecnologia (FCT, Portugal). Ana Isabel Bourbon acknowledges funding by FCT through the individual scientific employment program contract (2020.03447.CEECIND). This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit, and by LABBELS \u2013 Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020. We also would like to thank the Advanced Electron Microscopy, Imaging, and Spectroscopy (AEMIS) and Nanophotonics and Bioimaging (NBI) from INL for their support.

Available from: 2025-01-08 Created: 2025-01-08 Last updated: 2025-01-13Bibliographically approved
Sar, T., Ferreira, J. & Taherzadeh, M. J. (2024). A study on the use of olive oil mill wastewater to produce protein-rich fungal biomass. In: : . Paper presented at Innovations in Food Loss and Waste Management.
Open this publication in new window or tab >>A study on the use of olive oil mill wastewater to produce protein-rich fungal biomass
2024 (English)Conference paper, Poster (with or without abstract) (Refereed)
Abstract [en]

While olive oil is an important food product in the Mediterranean Sea Basin, olive oil mill wastewater (OOMW) and olive pomace, which are by-products of olive oil, are released in excessive quantities. OMWW is an important source of environmental pollutants due to its slightly acidic pH, high contents of phenol and chemical oxygen demand (COD). To overcome this problem, it was aimed to investigate the potential use of OOMW as an alternative substrate for biomass production by filamentous fungi in this study. For the cultivation, three edible fungi (Aspergillus oryzae CBS 819.72, Neurospora intermedia CBS 131.92, and Rhizopus delemar CBS 145940) were tested. Among them, A. oryzae was found to be a promising fungus in biomass production containing 14.9% protein. The protein content of the biomass was improved to 44.9% (w/w) by adding a nitrogen source (sodium nitrate) and removing the suspended solids. Concomitantly, 35-44% of COD reduction was also obtained after the fungal cultivation. Thus, the potential use of olive oil mill wastewater for the cultivation of fungal biomass was determined and at the same time, its pre-treatment was provided. However, the content of the obtained fungal biomass should be determined and its usability as feed should be investigated.

Keywords
Valorization, bioconversion, single cell protein, wastewater treatment.
National Category
Industrial Biotechnology
Identifiers
urn:nbn:se:hb:diva-31503 (URN)
Conference
Innovations in Food Loss and Waste Management
Available from: 2024-01-31 Created: 2024-01-31 Last updated: 2024-04-09Bibliographically approved
Parchami, M., Rustas, B.-O., Taherzadeh, M. J. & Mahboubi, A. (2024). An in vitro evaluation of partial energy replacement in a total mixed ration with volatile fatty acids derived from agro-industrial residues. Systems Microbiology and Biomanufacturing
Open this publication in new window or tab >>An in vitro evaluation of partial energy replacement in a total mixed ration with volatile fatty acids derived from agro-industrial residues
2024 (English)In: Systems Microbiology and Biomanufacturing, ISSN 2662-7655Article in journal (Refereed) Epub ahead of print
Abstract [en]

The scientific interest in volatile fatty acids (VFAs) as an energy source and chemical precursor in ruminant diets has been longstanding, as it has significant implications for animal physiology and well-being. The present study explores the substitution of volatile fatty acids (VFAs) derived from agro-food residues via acidogenic fermentation as an alternative energy source in ruminant feed. Utilizing the gas production method, rumen digestibility assays were conducted, wherein the recovered VFA effluent from the acidogenic fermentation of apple pomace and potato protein liquor was substituted for 10%, 20%, and 30% of the total mixed ration (TMR) energy. Various parameters such as gas, VFA yield and composition, VFA peak intervals, changes in pH, and ammonium nitrogen content were investigated. Based on the results obtained, provision of 20% and 30% of the energy with VFAs did not increase methane production or did not cause significant pH alternations. Nevertheless, such supplementation resulted in increased production and accumulation of VFAs in the rumen media. The bioconversion of agro-food side streams into VFAs opens a new path in sustainable nutrient recovery and feed production from low value agro-industrial residues.

Keywords
Acidogenic fermentation, Gas production method, Ruminant feed, Total mixed ration, Volatile fatty acids
National Category
Bioprocess Technology
Research subject
Resource Recovery
Identifiers
urn:nbn:se:hb:diva-32012 (URN)10.1007/s43393-024-00278-4 (DOI)001235458500001 ()2-s2.0-85194699199 (Scopus ID)
Funder
Swedish Research Council Formas, 2021-02458
Available from: 2024-06-10 Created: 2024-06-10 Last updated: 2024-10-01Bibliographically approved
Awasthi, M. K., Amobonye, A., Bhagwat, P., Ashokkumar, V., Gowd, S. C., Dregulo, A. M., . . . Taherzadeh, M. J. (2024). Biochemical engineering for elemental sulfur from flue gases through multi-enzymatic based approaches – A review. Science of the Total Environment, 914, Article ID 169857.
Open this publication in new window or tab >>Biochemical engineering for elemental sulfur from flue gases through multi-enzymatic based approaches – A review
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2024 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 914, article id 169857Article, review/survey (Refereed) Published
Abstract [en]

Flue gases are the gases which are produced from industries related to chemical manufacturing, petrol refineries, power plants and ore processing plants. Along with other pollutants, sulfur present in the flue gas is detrimental to the environment. Therefore, environmentalists are concerned about its removal and recovery of resources from flue gases due to its activation ability in the atmosphere to transform into toxic substances. This review is aimed at a critical assessment of the techniques developed for resource recovery from flue gases. The manuscript discusses various bioreactors used in resource recovery such as hollow fibre membrane reactor, rotating biological contractor, sequential batch reactor, fluidized bed reactor, entrapped cell bioreactor and hybrid reactors. In conclusion, this manuscript provides a comprehensive analysis of the potential of thermotolerant and thermophilic microbes in sulfur removal. Additionally, it evaluates the efficacy of a multi-enzyme engineered bioreactor in this process. Furthermore, the study introduces a groundbreaking sustainable model for elemental sulfur recovery, offering promising prospects for environmentally-friendly and economically viable sulfur removal techniques in various industrial applications. 

National Category
Energy Engineering
Research subject
Resource Recovery
Identifiers
urn:nbn:se:hb:diva-31440 (URN)10.1016/j.scitotenv.2023.169857 (DOI)001163983800001 ()2-s2.0-85182518097 (Scopus ID)
Available from: 2024-01-25 Created: 2024-01-25 Last updated: 2024-10-01Bibliographically approved
García Fernández, B., Staicu, L. C. & Taherzadeh, M. J. (2024). BOOK OF ABSTRACTS - WORKSHOP: MATCHING DIVERSE FEEDSTOCKS FOR BIOCHEMICAL RECOVERY: IMPACT OF THEIR QUALITY. Borås: Högskolan i Borås
Open this publication in new window or tab >>BOOK OF ABSTRACTS - WORKSHOP: MATCHING DIVERSE FEEDSTOCKS FOR BIOCHEMICAL RECOVERY: IMPACT OF THEIR QUALITY
2024 (English)Book (Other academic)
Place, publisher, year, edition, pages
Borås: Högskolan i Borås, 2024. p. 51
National Category
Bioprocess Technology
Research subject
Resource Recovery
Identifiers
urn:nbn:se:hb:diva-32388 (URN)978-91-89833-53-1 (ISBN)
Projects
COST Action FULLRECO4US
Available from: 2024-08-19 Created: 2024-08-19 Last updated: 2024-08-19Bibliographically approved
Oladzad, S., Fallah, N., Mahboubi, A., Afsham, N., Taherzadeh, M. J. & Toghyani, J. (2024). Comparison of acid and hydrothermal pretreatments of date waste for value creation. Scientific Reports, 14, 1-14, Article ID 18056.
Open this publication in new window or tab >>Comparison of acid and hydrothermal pretreatments of date waste for value creation
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2024 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 14, p. 1-14, article id 18056Article in journal (Refereed) Published
Abstract [sv]

The production of date syrup yields a substantial amount of date press cake (DPC), fibrous and moisturising material with great potential for generating value through bioprocessing. However, the recalcitrant structure of DPC affects the yield of products in bioprocesses. To boost the accessibility of the structure as well as increase the soluble fraction of carbohydrates and facilitate further enzymatic hydrolysis, hydrothermal and dilute acid (0.5% (v/v) sulfuric acid) pretreatments as cost-effective and feasible methods were applied on DPC at relatively low temperatures (80, 100, 120 and 140 degrees C) and reaction times (60 and 90 min). The success in pretreatment was then evaluated by a post-enzymatic treatment using an enzyme cocktail of cellulases and hemicelluloses. Based on total accessible sugar with minimum produced inhibitors, an optimal operating condition was considered acid pretreatment at 120 degrees C for 90 min with a 55.02% increase in total sugar yield. To explore the potential use of pretreated DPC, an anaerobic digestion was conducted on untreated and acid-pretreated DPC at 120 degrees C for 90 min. The results showed that pretreatment increased the total bioproduct yield, including hydrogen, ethanol, and volatile fatty acid yields, by 59.75%. This demonstrates the significant impact of pretreatment on product yields in a bioprocess. 

Place, publisher, year, edition, pages
Springer Nature, 2024
Keywords
Acid pretreatment, Anaerobic digestion, Date press cake, Enzymatic hydrolysis, Fermentable sugar, Hydrothermal pretreatment
National Category
Bioprocess Technology Food Engineering
Research subject
Resource Recovery
Identifiers
urn:nbn:se:hb:diva-32395 (URN)10.1038/s41598-024-68879-6 (DOI)001284942100068 ()2-s2.0-85200490445 (Scopus ID)
Available from: 2024-08-20 Created: 2024-08-20 Last updated: 2024-10-01Bibliographically approved
Parchami, M., Rustas, B.-O., Taherzadeh, M. J. & Mahboubi, A. (2024). Effect of Agro-Industrial by Products Derived from Volatile Fatty Acids on Ruminant Feed In Vitro Digestibility. Animals, 14(16), Article ID 2330.
Open this publication in new window or tab >>Effect of Agro-Industrial by Products Derived from Volatile Fatty Acids on Ruminant Feed In Vitro Digestibility
2024 (English)In: Animals, E-ISSN 2076-2615, Vol. 14, no 16, article id 2330Article in journal (Refereed) Published
Abstract [en]

The growing demand for sustainable ruminant feed alternatives has motivated the application of bioconversion approaches for the valorization of agro-food byproducts (AFB) into feed additives and supplements. The present study thoroughly investigated substituting volatile fatty acids (VFAs) obtained from acidogenic fermentation (AF) of AFB as an energy source in ruminant feed. Rumen in vitro digestibility assays were conducted utilizing the gas production method, wherein the VFAs obtained from AF of apple pomace and potato protein liquor was substituted with partial silage and concentrate energy at levels of 10%, 20%, and 30%. The results indicate that substituting 20% of the concentrate’s energy with VFA mixture significantly reduced methane production and had no adverse effect on the production and accumulation of VFAs in the simulated rumen media. Conversely, replacing 10% of the silage energy with VFAs led to a decrease in methane production and further enhanced the production of VFAs. Readily digestible VFAs in ruminant feed have the potential to enhance energy availability and sustainability in ruminant farming practices, aligning with the principles of circular economy and waste valorization. 

Keywords
acidogenic fermentation, agro-food byproducts, membrane bioreactor, ruminant feed alternative, sustainability
National Category
Industrial Biotechnology Animal and Dairy Science
Research subject
Resource Recovery
Identifiers
urn:nbn:se:hb:diva-32514 (URN)10.3390/ani14162330 (DOI)001305709900001 ()2-s2.0-85202624830 (Scopus ID)
Funder
Vinnova, 2020-03664Swedish Research Council Formas, 2021-02458
Available from: 2024-09-09 Created: 2024-09-09 Last updated: 2024-11-06Bibliographically approved
Saini, R., Tiwari, B. R., Brancoli, P., Taherzadeh, M. J. & Kaur Brar, S. (2024). Environmental assessment of Rhodosporidium toruloides-1588 based oil production using wood hydrolysate and crude glycerol. Bioresource Technology, 393, Article ID 130102.
Open this publication in new window or tab >>Environmental assessment of Rhodosporidium toruloides-1588 based oil production using wood hydrolysate and crude glycerol
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2024 (English)In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 393, article id 130102Article in journal (Refereed) Published
Abstract [en]

Rhodosporidium toruloides, an oleaginous yeast, is a potential feedstock for biodiesel production due to its ability to utilize lignocellulosic biomass-derived hydrolysate with a considerably high lipid titer of 50–70 % w/w. Hence, for the first-time environmental assessment of large-scale R. toruloides-based biodiesel production from wood hydrolysate and crude glycerol was conducted. The global warming potential was observed to be 0.67 kg CO2 eq./MJ along with terrestrial ecotoxicity of 1.37 kg 1,4-DCB eq./MJ and fossil depletion of 0.13 kg oil eq./MJ. The highest impacts for global warming (∼45 %) and fossil depletion (∼37 %) are attributed to the use of chloroform for lipid extraction while fuel consumption for transportation contributed more than 50 % to terrestrial ecotoxicity. Further, sensitivity analysis revealed that maximizing biodiesel yield by increasing lipid yield and solid loading could contribute to reduced environmental impacts. In nutshell, this investigation reveals that environmental impact varies with the type of chemical utilized.

Keywords
FFermentation Life cycle assessment, Sensitivity analysis, Oleaginous yeast, Uncertainty analysis
National Category
Bioenergy
Research subject
Resource Recovery
Identifiers
urn:nbn:se:hb:diva-31313 (URN)10.1016/j.biortech.2023.130102 (DOI)001135656300001 ()2-s2.0-85179471946 (Scopus ID)
Note

The authors are thankful for the funding and support from MITACS Globalink Research Award (Application number: IT22271), Natural sciences and engineering Canada (Strategic grant 506346) and James and Joanne Love Chair in Environmental Engineering at York University, Canada.

Available from: 2024-01-15 Created: 2024-01-15 Last updated: 2024-02-01Bibliographically approved
Dos Santos Neto, A., Wainaina, S., Chandolias, K., Piatek, P. & Taherzadeh, M. J. (2024). Exploring the Potential of Syngas Fermentation for Recovery of High-Value Resources: A Comprehensive Review. Current Pollution Reports, 11(1), Article ID 7.
Open this publication in new window or tab >>Exploring the Potential of Syngas Fermentation for Recovery of High-Value Resources: A Comprehensive Review
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2024 (English)In: Current Pollution Reports, E-ISSN 2198-6592, Vol. 11, no 1, article id 7Article, review/survey (Refereed) Published
Abstract [en]

Synthesis gas (syngas) fermentation represents a promising biological method for converting industrial waste gases, particularly carbon monoxide (CO) and carbon dioxide (CO2) from industrial sources (e.g. steel production or municipal waste gasification), into high-value products such as biofuels, chemicals, and animal feed using acetogenic bacteria. This review identifies and addresses key challenges that hinder the large-scale adoption of this technology, including limitations in gas mass transfer, an incomplete understanding of microbial metabolic pathways, and suboptimal bioprocess conditions. Our findings emphasize the critical role of microbial strain selection and bioprocess optimization to enhance productivity and scalability, with a focus on utilizing diverse microbial consortia and efficient reactor systems. By examining recent advancements in microbial conditioning, operational parameters, and reactor design, this study provides actionable insights to improve syngas fermentation efficiency, suggesting pathways towards overcoming current technical barriers for its broader industrial application beyond the production of bulk chemicals.

Keywords
Syngas fermentation, Mixed bacteria, BiofuelsIndustrial scale, Conditioning methods, Process development
National Category
Microbiology
Research subject
Resource Recovery
Identifiers
urn:nbn:se:hb:diva-32873 (URN)10.1007/s40726-024-00337-3 (DOI)001359470000001 ()
Available from: 2024-12-03 Created: 2024-12-03 Last updated: 2024-12-04Bibliographically approved
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ORCID iD: ORCID iD iconorcid.org/0000-0003-4887-2433

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